defined_medium_for_sulfate_reducing_bacteria_with_butyrate

CultureMech:003054 bacterial DEFINED LIQUID
Original name: DEFINED MEDIUM FOR SULFATE-REDUCING BACTERIA WITH BUTYRATE

Overview

External ontology
mediadive.medium:J708 (source link unavailable) — DEFINED MEDIUM FOR SULFATE-REDUCING BACTERIA WITH BUTYRATE
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=708

Composition

flowchart LR
    CultureMech_003054["defined_medium_for_sulfate_reducing_bacteria_with_butyrate"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003054 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003054 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003054 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003054 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_003054 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Resazurin["Resazurin"]
    CultureMech_003054 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Sodium_butyrate["Sodium butyrate"]
    CultureMech_003054 --> ing6_Sodium_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing6_Sodium_butyrate -.-> CHEBI_64103
    ing7_NaHCO3["NaHCO3"]
    CultureMech_003054 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003054 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_HCl["HCl"]
    CultureMech_003054 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003054 --> ing10_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing11_ZnCl2["ZnCl2"]
    CultureMech_003054 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003054 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_003054 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003054 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003054 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003054 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003054 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.499002 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.998004 G_PER_L CHEBI:31206
ammonium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0998004 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.49501 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NaCl
Cl.Na 58.443 g/mol
0.998004 G_PER_L CHEBI:26710
sodium chloride
6 Resazurin
C12H7NO4 229.191 g/mol
0.000998004 G_PER_L CHEBI:8806
Resazurin
7 Sodium butyrate
C4H7O2.Na 110.088 g/mol
100 G_PER_L CHEBI:64103
sodium butyrate
8 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
11 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients and adjust pH to 7.5. Distribute the medium into culture vessels under a N2-CO2 (95:5, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized) to the medium:
  2. Readjust pH of the medium to 7.5, if necessary.
  3. For preparation of solid medium, add 15.0 g/L agar (BD-Difco).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.361517Z mediadive-import Imported from MediaDive Source: JCM, ID: J708
2026-03-13T06:19:17.468516Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003054
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.178233+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DEFINED MEDIUM FOR SULFATE-REDUCING BACTERIA WITH BUTYRATE" to "defined_medium_for_sulfate_reducing_bacteria_with_butyrate" for consistent matching
2026-06-05T07:40:55.547072+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.178375+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.