Overview
- External ontology
- mediadive.medium:J724 (source link unavailable) — RAVOT MODIFIED MEDIUM FOR MARINITOGA LITORALIS
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=724
Composition
flowchart LR
CultureMech_003068["ravot_modified_medium_for_marinitoga_litoralis"]
ing0_NaCl["NaCl"]
CultureMech_003068 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003068 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_003068 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NH4Cl["NH4Cl"]
CultureMech_003068 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_003068 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003068 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
CultureMech_003068 --> ing6_Sodium_acetate_x_3_H2O
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
ing7_Yeast_extract["Yeast extract"]
CultureMech_003068 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_003068 --> ing8_Trypticase_peptone
ing9_Maltose["Maltose"]
CultureMech_003068 --> ing9_Maltose
CHEBI_17306["CHEBI:17306"]
ing9_Maltose -.-> CHEBI_17306
ing10_PIPES["PIPES"]
CultureMech_003068 --> ing10_PIPES
CHEBI_39033["CHEBI:39033"]
ing10_PIPES -.-> CHEBI_39033
ing11_Sulfur["Sulfur"]
CultureMech_003068 --> ing11_Sulfur
CHEBI_26833["CHEBI:26833"]
ing11_Sulfur -.-> CHEBI_26833
ing12_Resazurin["Resazurin"]
CultureMech_003068 --> ing12_Resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Resazurin -.-> CHEBI_8806
ing13_K2HPO4["K2HPO4"]
CultureMech_003068 --> ing13_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing13_K2HPO4 -.-> CHEBI_131527
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003068 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25.5152 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.490677 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.490677 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.294406 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4.3981354 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
[Merged 2 duplicates: 0.0981354, 4.3] |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0981354 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
|
0.814524 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 | Yeast extract | 1.96271 G_PER_L | ||
| 9 | Trypticase peptone | 1.96271 G_PER_L | ||
| 10 |
Maltose
C12H22O11 342.297 g/mol
|
1.96271 G_PER_L |
CHEBI:17306
maltose
|
|
| 11 | PIPES | 3.23847 G_PER_L |
CHEBI:39033
PIPES
|
|
| 12 |
Sulfur
S 32.067 g/mol
|
4.90677 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 13 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000981354 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 14 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Mix components except sulfur, adjust pH to 6.0 and autoclave under a N2 atmosphere. After cooling, add per liter the following solutions (autoclaved and stored anaerobically):
- Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2 atmosphere, and seal with butyl rubber stoppers. Then, add the following solution (autoclaved and stored under a N2 atmosphere) to the medium:
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.408164Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J724 |
| 2026-03-13T03:09:22.572423Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.564259Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003068 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.294558+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RAVOT MODIFIED MEDIUM FOR MARINITOGA LITORALIS" to "ravot_modified_medium_for_marinitoga_litoralis" for consistent matching |
| 2026-06-05T07:41:43.829708+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.839766+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |