rye_bran_mrs (CultureMech:003075)

Overview

External ontology

mediadive.medium:J730 (source link unavailable) — RYE-BRAN MRS

Applications

Microbial cultivation

Notes

MediaDive JCM J730 and the merged TOGO M753 copy list Rye-Bran MRS as Lactobacilli MRS broth in a medium containing 900 mL/L Rye-bran extract and 100 mL/L distilled water; the Rye-bran extract contains rye-bran, malted wheat meal, trypsin, and distilled water.

Composition

flowchart LR
    CultureMech_003075["rye_bran_mrs"]
    ing0_Lactobacilli_MRS_broth["Lactobacilli MRS broth"]
    CultureMech_003075 --> ing0_Lactobacilli_MRS_broth
    ing1_Distilled_water["Distilled water"]
    CultureMech_003075 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Lactobacilli MRS broth	55 G_PER_L		MediaDive solution 4656 lists 55 g/L Lactobacilli MRS broth.
2	Distilled water H2O 18.015 g/mol	100.0 ML_PER_L	CHEBI:15377 water	TOGO M753 lists distilled water.

Preparation

Prepare Rye-Bran MRS from Lactobacilli MRS broth, 900 mL Rye-bran extract, and 100 mL distilled water.
For the Rye-bran extract, mix the extract components and incubate at 50 C for 24 h.
Clarify the Rye-bran extract by centrifugation at 500 x g for 15 min followed by paper filtration.

Solutions

Rye-bran extract

Dose: 900.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Rye-bran	40 G_PER_L	MediaDive solution 4657 lists 40 g/L rye-bran.
Malted wheat meal	2 G_PER_L	MediaDive solution 4657 lists 2 g/L malted wheat meal.
Trypsin	0.8 G_PER_L	MediaDive solution 4657 lists 0.8 g/L trypsin.
Distilled water	1000 ML_PER_L	MediaDive JCM J730 Rye-bran extract lists distilled water.

The merged TOGO M753 copy lists 900 mL Rye-bran extract.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.424434Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J730
2026-03-13T06:19:17.607214Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003075
2026-03-17T19:45:31.345241+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "RYE-BRAN MRS" to "rye_bran_mrs" for consistent matching
2026-09-08T00:00:00-07:00	repair_jcm_rye_bran_mrs.py	RESOLVED_JCM_J730_RYE_BRAN_MRS_GRAPH	MediaDive JCM J730 and the merged TOGO M753 copy list Rye-Bran MRS as Lactobacilli MRS broth in a medium containing 900 mL/L Rye-bran extract and 100 mL/L distilled water; the Rye-bran extract contains rye-bran, malted wheat meal, trypsin, and distilled water.