bm_for_syntrophicus_schinkii

CultureMech:003080 bacterial COMPLEX LIQUID
Original name: BM FOR SYNTROPHICUS SCHINKII

Overview

External ontology
mediadive.medium:J737 (source link unavailable) — BM FOR SYNTROPHICUS SCHINKII
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=737

Composition

flowchart LR
    CultureMech_003080["bm_for_syntrophicus_schinkii"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003080 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_NaCl["NaCl"]
    CultureMech_003080 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003080 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003080 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_003080 --> ing4_Yeast_extract
    ing5_Resazurin["Resazurin"]
    CultureMech_003080 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003080 --> ing6_Trace_vitamins__see_Medium_No__197_
    ing7_NaHCO3["NaHCO3"]
    CultureMech_003080 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Sodium_lactate["L-Sodium lactate"]
    CultureMech_003080 --> ing8_L_Sodium_lactate
    CHEBI_232798["CHEBI:232798"]
    ing8_L_Sodium_lactate -.-> CHEBI_232798
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003080 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_KH2PO4["KH2PO4"]
    CultureMech_003080 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_Na2HPO4["Na2HPO4"]
    CultureMech_003080 --> ing11_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing11_Na2HPO4 -.-> CHEBI_34683
    ing12_HCl["HCl"]
    CultureMech_003080 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003080 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003080 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003080 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_ZnCl2["ZnCl2"]
    CultureMech_003080 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_H3BO3["H3BO3"]
    CultureMech_003080 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003080 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003080 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003080 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_AlCl3["AlCl3"]
    CultureMech_003080 --> ing21_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing21_AlCl3 -.-> CHEBI_30114
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003080 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_NaOH["NaOH"]
    CultureMech_003080 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003080 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.847458 G_PER_L CHEBI:31206
ammonium chloride
2 NaCl
Cl.Na 58.443 g/mol
0.847458 G_PER_L CHEBI:26710
sodium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0997009 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.279163 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 Yeast extract 0.199402 G_PER_L
6 Resazurin
C12H7NO4 229.191 g/mol
0.000498504 G_PER_L CHEBI:8806
Resazurin
7 Trace vitamins (see Medium No. 197) 1 G_PER_L
8 NaHCO3
CHO3.Na 84.006 g/mol
40 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Sodium lactate
C3H5O3.Na 112.06 g/mol
100 G_PER_L CHEBI:232798
sodium L-lactate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 KH2PO4
H2O4P.K 136.084 g/mol
4.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 Na2HPO4
HO4P.2Na 141.958 g/mol
4.3 G_PER_L CHEBI:34683
disodium hydrogenphosphate
13 HCl
HCl 36.461 g/mol
3.16832 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.9802 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.247525 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0990099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.0693069 G_PER_L CHEBI:49976
zinc dichloride
18 H3BO3
H3BO3 61.833 g/mol
0.00594059 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.039604 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0693069 G_PER_L CHEBI:34887
nickel dichloride
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0019802 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 AlCl3
AlCl3 133.341 g/mol
0.0247525 G_PER_L CHEBI:30114
aluminium trichloride
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.01394059 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 [Merged 2 duplicates: 0.00594059, 0.008]
24 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
25 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Mix components under a N2-CO2 (80:20, v/v) gas mixture and autoclave under the same gas mixture in bottles sealed with butyl rubber stoppers. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized) to the medium:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:17.439931Z mediadive-import Imported from MediaDive Source: JCM, ID: J737
2026-03-13T03:09:07.958358Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:17.645900Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003080
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.389187+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BM FOR SYNTROPHICUS SCHINKII" to "bm_for_syntrophicus_schinkii" for consistent matching
2026-06-05T07:40:51.193383+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.933317+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.