Overview
- External ontology
- mediadive.medium:J740 (source link unavailable) — MVL MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 740, MediaDive J740, and TOGO M765 describe MVL Medium as a 1.221 L main solution containing 1.0 L distilled water, 75.0 ml each of Mineral solution 1 and Mineral solution 2, 1.0 ml of 0.1% resazurin, 50.0 ml of 8% Na2CO3, 10.0 ml each of 0.07% hemin and 3% L-Cysteine HCl x H2O stocks, Trypticase peptone, yeast extract, beef extract, glucose, and agar.
Composition
flowchart LR
CultureMech_003083["mvl_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_003083 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003083 --> ing1_Yeast_extract
ing2_Beef_extract["Beef extract"]
CultureMech_003083 --> ing2_Beef_extract
ing3_Agar["Agar"]
CultureMech_003083 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Glucose["Glucose"]
CultureMech_003083 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Distilled_water["Distilled water"]
CultureMech_003083 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 8.19001 G_PER_L | MediaDive J740 / JCM Medium 740 lists 8.19001 g/L Trypticase peptone. | |
| 2 | Yeast extract | 4.095 G_PER_L |
FOODON:03315426
yeast extract
|
MediaDive J740 / JCM Medium 740 lists 4.095 g/L Yeast extract. |
| 3 | Beef extract | 1.638 G_PER_L | MediaDive J740 / JCM Medium 740 lists 1.638 g/L Beef extract. | |
| 4 | Agar | 12.285 G_PER_L |
CHEBI:2509
agar
|
MediaDive J740 / JCM Medium 740 lists 12.285 g/L Agar. |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
1.638 G_PER_L |
CHEBI:17234
glucose
|
MediaDive J740 / JCM Medium 740 lists 1.638 g/L Glucose. |
| 6 |
Distilled water
H2O 18.015 g/mol
|
819.001 ML_PER_L |
CHEBI:15377
water
|
MediaDive J740 / JCM Medium 740 contributes 819.001 ml/L distilled water. |
Preparation
- Prepare the main MVL Medium from 819.001 ml/L distilled water, 61.4251 ml/L each of Mineral solution 1 and Mineral solution 2, 0.819001 ml/L 0.1% Resazurin solution, 40.95 ml/L 8% Na2CO3 solution, 8.19001 ml/L each of 0.07% Hemin solution and 3% L-Cysteine HCl x H2O solution, Trypticase peptone, yeast extract, beef extract, glucose, and agar.
- Autoclave at 121 C for 15 min unless otherwise stated.
Solutions
Mineral solution 1
Dose: 61.4251 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 0.6 PERCENT_W_V | MediaDive J740 / JCM Medium 740 describes Mineral solution 1 as 0.6% KH2PO4. |
MediaDive J740 / JCM Medium 740 adds 61.4251 ml/L Mineral solution 1.
Mineral solution 2
Dose: 61.4251 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 | 6.0 G_PER_L | MediaDive J740 / JCM Medium 740 lists 6.0 g/L K2HPO4. |
| (NH4)2SO4 | 12.0 G_PER_L | MediaDive J740 / JCM Medium 740 lists 12.0 g/L (NH4)2SO4. |
| NaCl | 12.0 G_PER_L | MediaDive J740 / JCM Medium 740 lists 12.0 g/L NaCl. |
| MgSO4 x 7 H2O | 1.2 G_PER_L | MediaDive J740 / JCM Medium 740 lists 1.2 g/L MgSO4 x 7 H2O. |
| CaCl2 x 2 H2O | 1.2 G_PER_L | MediaDive J740 / JCM Medium 740 lists 1.2 g/L CaCl2 x 2 H2O. |
| Distilled water | 1000.0 ML_PER_L | MediaDive J740 / JCM Medium 740 makes Mineral solution 2 up with 100.0 ml water. |
MediaDive J740 / JCM Medium 740 adds 61.4251 ml/L Mineral solution 2.
0.1% Resazurin solution
Dose: 0.819001 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Resazurin | 0.1 PERCENT_W_V | MediaDive J740 / JCM Medium 740 specifies 0.1% Resazurin solution as 0.1% w/v. |
MediaDive J740 / JCM Medium 740 adds 0.819001 ml/L 0.1% Resazurin solution.
0.07% Hemin solution
Dose: 8.19001 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Hemin | 0.07 PERCENT_W_V | MediaDive J740 / JCM Medium 740 specifies 0.07% Hemin solution as 0.07% w/v. |
MediaDive J740 / JCM Medium 740 adds 8.19001 ml/L 0.07% Hemin solution.
8% Na2CO3 solution
Dose: 40.95 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | 8.0 PERCENT_W_V | MediaDive J740 / JCM Medium 740 specifies 8% Na2CO3 solution as 8.0% w/v. |
MediaDive J740 / JCM Medium 740 adds 40.95 ml/L 8% Na2CO3 solution.
3% L-Cysteine HCl x H2O solution
Dose: 8.19001 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl x H2O | 3.0 PERCENT_W_V | MediaDive J740 / JCM Medium 740 specifies 3% L-Cysteine HCl x H2O solution as 3.0% w/v. |
MediaDive J740 / JCM Medium 740 adds 8.19001 ml/L 3% L-Cysteine HCl x H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.447589Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J740 |
| 2026-03-13T06:19:17.659692Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003083 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.405095+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MVL MEDIUM" to "mvl_medium" for consistent matching |
| 2026-06-05T07:41:38.937632+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.311103+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m765_mvl_score15.py | RESOLVED_JCM_740_MVL_MEDIUM | Moved Mineral solution 1, Mineral solution 2, 0.1% Resazurin, 0.07% Hemin, 8% Na2CO3, and 3% L-Cysteine HCl x H2O stocks into nested solution entries, restored main distilled water, and linked the TOGO M765 source duplicate. |