nutrient_agar

CultureMech:003093 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT AGAR

Overview

External ontology
mediadive.medium:J74 (source link unavailable) — NUTRIENT AGAR
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 74 / Nutrient Agar lists 1.0 L distilled water, 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar, adjusted to pH 7.0; TOGO M65 and MediaDive J74 report the same JCM recipe.

Composition

flowchart LR
    CultureMech_003093["nutrient_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003093 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Peptone["Peptone"]
    CultureMech_003093 --> ing1_Peptone
    ing2_Beef_extract["Beef extract"]
    CultureMech_003093 --> ing2_Beef_extract
    ing3_Agar["Agar"]
    CultureMech_003093 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 MediaDive J74 / JCM Medium 74 lists 1.0 L Distilled water.
2 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 MediaDive J74 / JCM Medium 74 lists 5.0 g/L Peptone.
3 Beef extract 3.0 G_PER_L FOODON:03302088
Beef extract
 MediaDive J74 / JCM Medium 74 lists 3.0 g/L Beef extract.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 MediaDive J74 / JCM Medium 74 lists 15.0 g/L Agar.

Preparation

  1. Dissolve 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar in 1.0 L distilled water.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.683971Z mediadive-import Imported from MediaDive Source: JCM, ID: J74
2026-03-13T06:19:17.752207Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003093
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.511963+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT AGAR" to "nutrient_agar" for consistent matching
2026-06-05T07:40:17.668550+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m92_alkaline_nutrient_score15.py RESOLVED_MEDIADIVE_J74_NUTRIENT_AGAR Added source 1.0 L distilled water, grounded peptone and beef extract, retained the pH 7.0 adjustment, and linked the TOGO M65 source duplicate plus JCM Medium 100 alkaline variant.
2026-09-12T00:00:00-07:00 repair_nutrient_ncg_nssm_score15.py LINKED_JCM_1109_SUPPLEMENTED_VARIANT Linked JCM Medium 1109 / TOGO M1186 as the NaCl and yeast-extract supplemented variant of JCM Medium 74 Nutrient Agar.