dehalospirillum_medium

CultureMech:003096 bacterial COMPLEX LIQUID
Original name: DEHALOSPIRILLUM MEDIUM

Overview

External ontology
mediadive.medium:J753 (source link unavailable) — DEHALOSPIRILLUM MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=753

Composition

flowchart LR
    CultureMech_003096["dehalospirillum_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_003096 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003096 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003096 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003096 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003096 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_003096 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003096 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Resazurin["Resazurin"]
    CultureMech_003096 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003096 --> ing8_Yeast_extract
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003096 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003096 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_003096 --> ing11_Potassium_phosphate_buffer
    ing12_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003096 --> ing12_Trace_vitamins__see_Medium_No__197_
    ing13_NaHCO3["NaHCO3"]
    CultureMech_003096 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_003096 --> ing14_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing14_Sodium_pyruvate -.-> CHEBI_50144
    ing15_Sodium_fumarate["Sodium fumarate"]
    CultureMech_003096 --> ing15_Sodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing15_Sodium_fumarate -.-> CHEBI_115156
    ing16_HCl["HCl"]
    CultureMech_003096 --> ing16_HCl
    CHEBI_17883["CHEBI:17883"]
    ing16_HCl -.-> CHEBI_17883
    ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003096 --> ing17_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing18_ZnCl2["ZnCl2"]
    CultureMech_003096 --> ing18_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing18_ZnCl2 -.-> CHEBI_49976
    ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003096 --> ing19_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing20_H3BO3["H3BO3"]
    CultureMech_003096 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003096 --> ing21_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003096 --> ing22_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003096 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003096 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_NaOH["NaOH"]
    CultureMech_003096 --> ing25_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing25_NaOH -.-> CHEBI_32145
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003096 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003096 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
0.683594 G_PER_L CHEBI:32149
sodium sulfate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.195312 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.244141 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
0.244141 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.390625 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.488281 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.146484 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000488281 G_PER_L CHEBI:8806
Resazurin
9 Yeast extract 1.95312 G_PER_L
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.0488281 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0244141 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 Potassium phosphate buffer 10 G_PER_L ( pH 7.5)
13 Trace vitamins (see Medium No. 197) 10 G_PER_L
14 NaHCO3
CHO3.Na 84.006 g/mol
42 G_PER_L CHEBI:32139
sodium hydrogencarbonate
15 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
18 G_PER_L CHEBI:50144
sodium pyruvate
16 Sodium fumarate
C4H2O4.2Na 160.036 g/mol
40 G_PER_L CHEBI:115156
disodium fumarate
17 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
18 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
19 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
20 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
22 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
23 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components except L-cysteine x HCl x H2O and FeSO4 x 7H2O. Bring to a boil for a few seconds and cool down under a N2 atmosphere. While gassing with N2, add L-cysteine x HCl x H2O and FeSO4 x 7H2O to the solution, distribute into culture vessels (e.g., 18 ml in 50 ml serum bottles) under a N2 atmosphere, then, seal butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter (final) the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks:
  2. Adjust pH of the medium to 7.3 - 7.7, if necessary.

Solutions

Vitamin B12 solution (see Medium No. 403)

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.489732Z mediadive-import Imported from MediaDive Source: JCM, ID: J753
2026-03-09T08:05:07.743728+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:17.776377Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003096
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.539878+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DEHALOSPIRILLUM MEDIUM" to "dehalospirillum_medium" for consistent matching
2026-05-16T09:02:59.338537+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:17.680325+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.094932+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.