alkaline_tryptone_soya_broth_medium

CultureMech:003106 bacterial COMPLEX LIQUID
Original name: ALKALINE TRYPTONE SOYA BROTH MEDIUM

Overview

External ontology
mediadive.medium:J763 (source link unavailable) — ALKALINE TRYPTONE SOYA BROTH MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
MediaDive J763 and TOGO M789 describe JCM Medium 763 Alkaline Tryptone Soya Broth Medium as 30.0 g/L Oxoid Tryptone soya broth in 1000.0 ml/L distilled water, adjusted after autoclaving to pH 8.5-9.0 with sterile autoclaved 20% sodium carbonate solution.

Composition

flowchart LR
    CultureMech_003106["alkaline_tryptone_soya_broth_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003106 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Tryptone_soya_broth__Oxoid_["Tryptone soya broth (Oxoid)"]
    CultureMech_003106 --> ing1_Tryptone_soya_broth__Oxoid_

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 MediaDive J763 / JCM Medium 763 contributes 1000.0 ml/L distilled water.
2 Tryptone soya broth (Oxoid) 30.0 G_PER_L MediaDive J763 / JCM Medium 763 lists 30.0 g/L Oxoid Tryptone soya broth.

Preparation

  1. Dissolve 30.0 g/L Oxoid Tryptone soya broth in 1000.0 ml/L water.
  2. Autoclave.
  3. After cooling, adjust pH to 8.5-9.0 with sterile autoclaved 20% sodium carbonate solution.

Solutions

20% sodium carbonate solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 20.0 PERCENT_W_V MediaDive J763 / JCM Medium 763 specifies the pH-adjusting sodium carbonate solution as 20.0% w/v.
MediaDive J763 / JCM Medium 763 uses sterile 20% sodium carbonate to adjust pH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.526507Z mediadive-import Imported from MediaDive Source: JCM, ID: J763
2026-03-13T06:19:17.862335Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003106
2026-03-17T19:45:31.637892+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALINE TRYPTONE SOYA BROTH MEDIUM" to "alkaline_tryptone_soya_broth_medium" for consistent matching
2026-09-11T00:00:00-07:00 repair_togo_m789_alkaline_tsb_score15.py RESOLVED_JCM_763_ALKALINE_TRYPTONE_SOYA_BROTH Restored source-level Oxoid Tryptone soya broth, removed the Wikipedia-derived constituent expansion and agar from the liquid parent, nested 20% sodium carbonate as the variable pH adjuster, added the final pH range, and linked the TOGO M789 and M790 child records.