betaine_bicarbonate_buffered_medium

CultureMech:003112 bacterial COMPLEX LIQUID
Original name: BETAINE+BICARBONATE BUFFERED MEDIUM

Overview

External ontology
mediadive.medium:J770 (source link unavailable) — BETAINE+BICARBONATE BUFFERED MEDIUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=770

Composition

flowchart LR
    CultureMech_003112["betaine_bicarbonate_buffered_medium"]
    ing0_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003112 --> ing0_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing0_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing1_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003112 --> ing1_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing1_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003112 --> ing2_Yeast_extract
    ing3_Resazurin["Resazurin"]
    CultureMech_003112 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Betaine["Betaine"]
    CultureMech_003112 --> ing4_Betaine
    CHEBI_17750["CHEBI:17750"]
    ing4_Betaine -.-> CHEBI_17750
    ing5_NaHCO3["NaHCO3"]
    CultureMech_003112 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003112 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003112 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_KH2PO4["KH2PO4"]
    CultureMech_003112 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_Na2HPO4["Na2HPO4"]
    CultureMech_003112 --> ing9_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing9_Na2HPO4 -.-> CHEBI_34683
    ing10_H3BO3["H3BO3"]
    CultureMech_003112 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_ZnCl2["ZnCl2"]
    CultureMech_003112 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003112 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003112 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003112 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_AlCl3["AlCl3"]
    CultureMech_003112 --> ing15_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing15_AlCl3 -.-> CHEBI_30114
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003112 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_NiCl2_x_H2O["NiCl2 x H2O"]
    CultureMech_003112 --> ing17_NiCl2_x_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_H2O -.-> CHEBI_34887
    ing18_EDTA["EDTA"]
    CultureMech_003112 --> ing18_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing18_EDTA -.-> CHEBI_4735
    ing19_HCl["HCl"]
    CultureMech_003112 --> ing19_HCl
    CHEBI_17883["CHEBI:17883"]
    ing19_HCl -.-> CHEBI_17883
    ing20_NH4Cl["NH4Cl"]
    CultureMech_003112 --> ing20_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing20_NH4Cl -.-> CHEBI_31206
    ing21_NaCl["NaCl"]
    CultureMech_003112 --> ing21_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing21_NaCl -.-> CHEBI_26710
    ing22_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003112 --> ing22_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing22_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing23_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003112 --> ing23_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing23_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing24_Biotin["Biotin"]
    CultureMech_003112 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_003112 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Pyridoxamine["Pyridoxamine"]
    CultureMech_003112 --> ing26_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing26_Pyridoxamine -.-> CHEBI_16410
    ing27_Riboflavin["Riboflavin"]
    CultureMech_003112 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinamide["Nicotinamide"]
    CultureMech_003112 --> ing28_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing28_Nicotinamide -.-> CHEBI_17154
    ing29_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003112 --> ing29_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_DL_Calcium_pantothenate -.-> CHEBI_31345
    CultureMech_003112 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.000327869 G_PER_L CHEBI:131361
disodium selenite pentahydrate
2 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.000327869 G_PER_L CHEBI:63939
sodium tungstate dihydrate
3 Yeast extract 0.218579 G_PER_L
4 Resazurin
C12H7NO4 229.191 g/mol
0.000546448 G_PER_L CHEBI:8806
Resazurin
5 Betaine
C5H11NO2 117.148 g/mol
10 G_PER_L CHEBI:17750
glycine betaine
6 NaHCO3
CHO3.Na 84.006 g/mol
80 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
9 KH2PO4
H2O4P.K 136.084 g/mol
4.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 Na2HPO4
HO4P.2Na 141.958 g/mol
4.3 G_PER_L CHEBI:34683
disodium hydrogenphosphate
11 H3BO3
H3BO3 61.833 g/mol
0.04995 G_PER_L CHEBI:33118
boric acid
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.04995 G_PER_L CHEBI:49976
zinc dichloride
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02997 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.499501 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.04995 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 AlCl3
AlCl3 133.341 g/mol
0.04995 G_PER_L CHEBI:30114
aluminium trichloride
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.04995 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 NiCl2 x H2O
Cl2Ni 129.599 g/mol
0.04995 G_PER_L CHEBI:34887
nickel dichloride
19 EDTA
C10H16N2O8 292.244 g/mol
0.499501 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
20 HCl
HCl 36.461 g/mol
1 G_PER_L CHEBI:17883
hydrogen chloride
21 NH4Cl
Cl.H4N 53.492 g/mol
24 G_PER_L CHEBI:31206
ammonium chloride
22 NaCl
Cl.Na 58.443 g/mol
24 G_PER_L CHEBI:26710
sodium chloride
23 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
8.8 G_PER_L CHEBI:86158
calcium chloride dihydrate
24 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
25 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.25 G_PER_L CHEBI:16410
pyridoxamine
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
29 Nicotinamide
C6H6N2O 122.127 g/mol
0.1 G_PER_L CHEBI:17154
nicotinamide
30 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
31 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
32 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
33 Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
  2. Mix components thoroughly and filter-sterilize. Stock the solution under a N2-CO2 (80:20, v/v) gas mixture.
  3. Mix components thoroughly and filter-sterilize. Stock the solution under a N2-CO2 (80:20, v/v) gas mixture.
  4. To complete the medium, combine Solutions A, B and C aseptically and anaerobically under a N2-CO2 (80:20, v/v) gas mixture. Check the final pH to be 6.9 - 7.2.

Solutions

Trace metal solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.998 G_PER_L

Vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Pyridoxine hydrochloride 0.1 G_PER_L
Thiamine HCl 0.1 G_PER_L
Nicotinic acid 0.1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.545406Z mediadive-import Imported from MediaDive Source: JCM, ID: J770
2026-03-13T06:19:17.900959Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003112
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.684572+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BETAINE+BICARBONATE BUFFERED MEDIUM" to "betaine_bicarbonate_buffered_medium" for consistent matching
2026-06-05T07:40:50.783459+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 36 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.864873+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.333910+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace metal solution' @ 1 ml/l; 3 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).