Overview
- External ontology
- mediadive.medium:J776 (source link unavailable) — MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM
- Applications
-
- Microbial cultivation
- pH
- 4.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=776
Composition
flowchart LR
CultureMech_003118["mh1_medium_for_saliarchaeum_rubrodurum"]
ing0_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_003118 --> ing0_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamic_acid -.-> CHEBI_16015
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003118 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_KH2PO4["KH2PO4"]
CultureMech_003118 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_003118 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2SO4["K2SO4"]
CultureMech_003118 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003118 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_NaCl["NaCl"]
CultureMech_003118 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Casamino_acids["Casamino acids"]
CultureMech_003118 --> ing7_Casamino_acids
ing8_Yeast_extract["Yeast extract"]
CultureMech_003118 --> ing8_Yeast_extract
ing9_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
CultureMech_003118 --> ing9_FeSO4_x_6_H2O
CHEBI_75832["CHEBI:75832"]
ing9_FeSO4_x_6_H2O -.-> CHEBI_75832
ing10_Agar["Agar"]
CultureMech_003118 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003118 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003118 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnCl2["ZnCl2"]
CultureMech_003118 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_H3BO4["H3BO4"]
CultureMech_003118 --> ing14_H3BO4
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003118 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003118 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003118 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_AlCl3["AlCl3"]
CultureMech_003118 --> ing18_AlCl3
CHEBI_30114["CHEBI:30114"]
ing18_AlCl3 -.-> CHEBI_30114
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003118 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003118 --> ing20_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
ing21_BaCl2_x_2_H2O["BaCl2 x 2 H2O"]
CultureMech_003118 --> ing21_BaCl2_x_2_H2O
CHEBI_86153["CHEBI:86153"]
ing21_BaCl2_x_2_H2O -.-> CHEBI_86153
ing22_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_003118 --> ing22_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing22_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
2 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
250.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 200.0, 50.0] |
| 8 | Casamino acids | 4 G_PER_L | ||
| 9 | Yeast extract | 2 G_PER_L | ||
| 10 |
FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
|
0.004 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 11 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.3 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 | H3BO4 | 0.02 G_PER_L | ||
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 22 |
BaCl2 x 2 H2O
Ba.2Cl.2H2O 244.264 g/mol
|
0.1 G_PER_L |
CHEBI:86153
barium chloride dihydrate
|
|
| 23 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH.
- For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
- Combine both solutions after autoclaving.
- Adjust pH to 4.0 with HCl.
Solutions
Trace metal solution
Dose: 2 G_PER_L
See mediadive_6140_Trace_metal_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.564248Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J776 |
| 2026-01-28T19:48:07.349281Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:11.852420+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:38.638461Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.944107Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003118 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.734008+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM" to "mh1_medium_for_saliarchaeum_rubrodurum" for consistent matching |
| 2026-05-16T09:04:23.351957+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:33.666212+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.137566+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:07.763502+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |