patel_laboratory_medium_with_glycerin

CultureMech:003123 bacterial COMPLEX LIQUID
Original name: PATEL LABORATORY MEDIUM WITH GLYCERIN

Overview

External ontology
mediadive.medium:J780 (source link unavailable) — PATEL LABORATORY MEDIUM WITH GLYCERIN
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 780 contains mineral salts, HEPES, 1.0 ml/L Trace vitamins from JCM Medium 197, 1.0 ml/L Trace mineral solution from JCM Medium 684, 0.2 g/L yeast extract, 2.84 g/L sodium sulfate, 1.0 L distilled water, and 10.0 ml/L 20% glycerin solution; the pH is adjusted to 7.0.

Composition

flowchart LR
    CultureMech_003123["patel_laboratory_medium_with_glycerin"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003123 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003123 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_003123 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003123 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003123 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_003123 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_HEPES["HEPES"]
    CultureMech_003123 --> ing6_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES -.-> CHEBI_46756
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_003123 --> ing7_Yeast_extract
    ing8_Na2SO4["Na2SO4"]
    CultureMech_003123 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_Distilled_water["Distilled water"]
    CultureMech_003123 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1.0 G_PER_L CHEBI:31206
ammonium chloride
 JCM Medium 780 lists 1.0 g/L.
2 K2HPO4
HO4P.2K 174.174 g/mol
0.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 780 lists 0.6 g/L.
3 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 780 lists 0.3 g/L.
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 JCM Medium 780 lists 0.1 g/L.
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 JCM Medium 780 lists 0.1 g/L.
6 NaCl
Cl.Na 58.443 g/mol
1.0 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 780 lists 1.0 g/L.
7 HEPES 12.0 G_PER_L CHEBI:46756
HEPES
 JCM Medium 780 lists 12.0 g/L.
8 Yeast extract 0.2 G_PER_L FOODON:03315426
yeast extract
 JCM Medium 780 lists 0.2 g/L.
9 Na2SO4
2Na.O4S 142.043 g/mol
2.84 G_PER_L CHEBI:32149
sodium sulfate
 JCM Medium 780 lists 2.84 g/L.
10 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 JCM Medium 780 lists 1000 ml/L.

Preparation

  1. Mix the salts, HEPES, trace solutions, yeast extract, sodium sulfate, and distilled water.
  2. Adjust pH to 7.0.
  3. Autoclave under a N2 atmosphere.
  4. After cooling, aseptically and anaerobically add 10.0 ml/L 20% glycerin solution.

Solutions

Trace vitamins

Dose: 1.0 ML_PER_L

JCM Medium 780 lists 1.0 ml/L Trace vitamins from JCM Medium 197.

Trace mineral solution

Dose: 1.0 ML_PER_L

JCM Medium 780 lists 1.0 ml/L Trace mineral solution from JCM Medium 684.

20% (w/v) Glycerin solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Glycerol 200.0 G_PER_L
JCM Medium 780 adds 10.0 ml/L 20% (w/v) Glycerin solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.579057Z mediadive-import Imported from MediaDive Source: JCM, ID: J780
2026-03-13T06:19:17.980104Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003123
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.774788+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PATEL LABORATORY MEDIUM WITH GLYCERIN" to "patel_laboratory_medium_with_glycerin" for consistent matching
2026-06-05T07:41:40.944401+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.903011+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-10T00:00:00-07:00 repair_jcm_780_score20.py RESOLVED_JCM_780_SCORE20_GRAPH JCM Medium 780 contains mineral salts, HEPES, 1.0 ml/L Trace vitamins from JCM Medium 197, 1.0 ml/L Trace mineral solution from JCM Medium 684, 0.2 g/L yeast extract, 2.84 g/L sodium sulfate, 1.0 L distilled water, and 10.0 ml/L 20% glycerin solution; the pH is adjusted to 7.0.