Overview
- External ontology
- mediadive.medium:J787 (source link unavailable) — HALOBACTERIUM TMM MEDIIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=787
Composition
flowchart LR
CultureMech_003130["halobacterium_tmm_mediium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_003130 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_003130 --> ing1_Yeast_extract
ing2_Na_glutamate["Na glutamate"]
CultureMech_003130 --> ing2_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing2_Na_glutamate -.-> CHEBI_64220
ing3_Trisodium_citrate["Trisodium citrate"]
CultureMech_003130 --> ing3_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate -.-> CHEBI_53258
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003130 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_003130 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaCl["NaCl"]
CultureMech_003130 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003130 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003130 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 0.5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
0.1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 4 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 MG_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 MG_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2.
- For preparation of solid medium, add 20.0 g/L Agar, Noble (BD-Difco).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.606190Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J787 |
| 2026-01-28T19:48:13.543181Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.050466Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003130 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.854032+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIUM TMM MEDIIUM" to "halobacterium_tmm_mediium" for consistent matching |
| 2026-06-05T07:41:07.615779+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.678055+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |