lind_8a_medium

CultureMech:003136 bacterial COMPLEX LIQUID
Original name: LIND 8A MEDIUM

Overview

External ontology
mediadive.medium:J792 (source link unavailable) — LIND 8A MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=792 Composition recovery source: https://togomedium.org/medium/M824 (TOGO snapshot of JCM medium J792).

Composition

flowchart LR
    CultureMech_003136["lind_8a_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003136 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003136 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_003136 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_003136 --> ing3_CaCl2_2H2O
    ing4_KH2PO4["KH2PO4"]
    CultureMech_003136 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_003136 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_003136 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_003136 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_003136 --> ing8_MgCl2_6H2O
    ing9_KCl["KCl"]
    CultureMech_003136 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_003136 --> ing10_L__Cysteine_HCl_H2O
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_003136 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_003136 --> ing12_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing12_Nitrogen_gas -.-> CHEBI_17997
    ing13_sodium_pyruvate["sodium pyruvate"]
    CultureMech_003136 --> ing13_sodium_pyruvate

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 CaCl2・2H2O 0.1 G_PER_L Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 Resazurin
C12H7NO4 229.191 g/mol
1 MG_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
9 MgCl2・6H2O 1 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 L--Cysteine・HCl・H2O 0.5 G_PER_L Properties: Defined component, Organic compound, Simple component
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
13 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
14 sodium pyruvate 20 MILLIMOLAR Role: Carbon source; Properties: Defined component, Organic compound, Solution

Preparation

  1. Mix components thoroughly, adjust pH to 7.0 with KOH, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2--CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter--sterilized):
  2. Use Medium No. [786], replacing glucose with sodium pyruvate (final 20 mM).

Solutions

Trace minerals (see Medium [M142])

Dose: 10 ML_PER_L

Defined in TOGO medium M142.

8% NaHCO3 solution*

Dose: 25 ML_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

5% Na2S・9H2O solution

Dose: 8 ML_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.618850Z mediadive-import Imported from MediaDive Source: JCM, ID: J792
2026-02-03T11:26:56.132545 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:18.081502Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003136
2026-03-17T19:45:31.889031+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LIND 8A MEDIUM" to "lind_8a_medium" for consistent matching
2026-04-05T17:20:25.527515 mark_unavailable_media MARKED_UNAVAILABLE Source database has no composition data. Category: EMPTY. JCM page returns "Nothing found"
2026-05-16T08:57:37.079449+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(2 entries)
2026-08-06T07:56:36.109079+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_jcm_missing_from_togo.py RECOVERED_JCM_COMPOSITION_FROM_TOGO Recovered JCM J792 from source-equivalent TOGO M824. Preserved final ingredients and stock-solution boundaries; did not flatten referenced media or stocks.
2026-08-26T02:13:03.001390+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 9 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).