Overview
- External ontology
- mediadive.medium:J796 (source link unavailable) — DESULFITOCELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=796
Composition
flowchart LR
CultureMech_003140["desulfitocella_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003140 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003140 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_003140 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_003140 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003140 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003140 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_KCl["KCl"]
CultureMech_003140 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Yeast_extract["Yeast extract"]
CultureMech_003140 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_003140 --> ing8_Trypticase_peptone
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003140 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Resazurin["Resazurin"]
CultureMech_003140 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_NaHCO3["NaHCO3"]
CultureMech_003140 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Sodium_sulfite["Sodium sulfite"]
CultureMech_003140 --> ing12_Sodium_sulfite
CHEBI_86477["CHEBI:86477"]
ing12_Sodium_sulfite -.-> CHEBI_86477
ing13_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003140 --> ing13_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing13_Sodium_pyruvate -.-> CHEBI_50144
ing14_Sodium_dithionite["Sodium dithionite"]
CultureMech_003140 --> ing14_Sodium_dithionite
CHEBI_66870["CHEBI:66870"]
ing14_Sodium_dithionite -.-> CHEBI_66870
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003140 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2997 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2997 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.999001 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.999001 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.499501 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0999001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.0999001 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 | Yeast extract | 0.999001 G_PER_L | ||
| 9 | Trypticase peptone | 0.999001 G_PER_L | ||
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.499501 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000999001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Sodium sulfite
2Na.O3S 126.044 g/mol
|
20 G_PER_L |
CHEBI:86477
sodium sulfite
|
|
| 14 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
20 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 15 |
Sodium dithionite
2Na.O4S2 174.11 g/mol
|
2 G_PER_L |
CHEBI:66870
sodium dithionite
|
|
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Mix components thoroughly, adjust pH to 7.0 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per 925 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
Solutions
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.629638Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J796 |
| 2026-03-09T08:04:26.364530+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:18.108214Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003140 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.918568+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFITOCELLA MEDIUM" to "desulfitocella_medium" for consistent matching |
| 2026-05-16T09:03:29.058778+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:56.062009+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.364221+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |