Overview
- External ontology
- mediadive.medium:J805 (source link unavailable) — 1/10 R2A BROTH
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 805 lists 0.32 g R2A broth DAIGO per 1.0 L distilled water; this is a 1:10 dilution of the JCM Medium 839 R2A Broth DAIGO premix.
Composition
flowchart LR
CultureMech_003150["1_10_r2a_broth"]
ing0_R2A_broth__DAIGO___Nihon_Pharm__Co__["R2A broth (DAIGO) (Nihon Pharm. Co.)"]
CultureMech_003150 --> ing0_R2A_broth__DAIGO___Nihon_Pharm__Co__
ing1_Distilled_water["Distilled water"]
CultureMech_003150 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | R2A broth (DAIGO) (Nihon Pharm. Co.) | 0.32 G_PER_L | JCM Medium 805 lists 0.32 g R2A broth DAIGO in 1.0 L distilled water. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 805 lists 1.0 L distilled water. |
Preparation
- Dissolve 0.32 g R2A broth (DAIGO) in 1.0 L distilled water.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.649526Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J805 |
| 2026-03-13T06:19:18.145594Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003150 |
| 2026-03-17T19:45:31.960642+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/10 R2A BROTH" to "1_10_r2a_broth" for consistent matching |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | RESOLVED_R2A_SCORE40_GRAPH | JCM Medium 805 lists 0.32 g R2A broth DAIGO per 1.0 L distilled water; this is a 1:10 dilution of the JCM Medium 839 R2A Broth DAIGO premix. |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | LINKED_R2A_SCORE40_CHILDREN | Added or refreshed bacterial/TOGO_M840_1_10_R2A_Broth.yaml as a child of bacterial/1_10_r2a_broth.yaml. |
| 2026-10-07T07:16:23Z | codex | CURATED_CONCENTRATION_EVIDENCE | Exact source snippets and claim-specific calculations attached in YAML. Batch volumes remain separate from stock strengths. Unresolved final-volume claims were not altered. |