Overview
- External ontology
- mediadive.medium:J808 (source link unavailable) — SULFURIMONAS GD1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=808
Composition
flowchart LR
CultureMech_003153["sulfurimonas_gd1_medium"]
ing0_NaCl["NaCl"]
CultureMech_003153 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003153 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003153 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_003153 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na2SO4["Na2SO4"]
CultureMech_003153 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_NaHCO3["NaHCO3"]
CultureMech_003153 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_KBr["KBr"]
CultureMech_003153 --> ing6_KBr
CHEBI_32030["CHEBI:32030"]
ing6_KBr -.-> CHEBI_32030
ing7_H3BO3["H3BO3"]
CultureMech_003153 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_003153 --> ing8_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing8_SrCl2_x_6_H2O -.-> CHEBI_36385
ing9_NH4Cl["NH4Cl"]
CultureMech_003153 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_KH2PO4["KH2PO4"]
CultureMech_003153 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_NaF["NaF"]
CultureMech_003153 --> ing11_NaF
CHEBI_28741["CHEBI:28741"]
ing11_NaF -.-> CHEBI_28741
ing12_HEPES["HEPES"]
CultureMech_003153 --> ing12_HEPES
CHEBI_46756["CHEBI:46756"]
ing12_HEPES -.-> CHEBI_46756
ing13_KNO3["KNO3"]
CultureMech_003153 --> ing13_KNO3
CHEBI_63043["CHEBI:63043"]
ing13_KNO3 -.-> CHEBI_63043
ing14_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_003153 --> ing14_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing14_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing15_HCl["HCl"]
CultureMech_003153 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003153 --> ing16_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
ing17_ZnCl2["ZnCl2"]
CultureMech_003153 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003153 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003153 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003153 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003153 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003153 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Biotin["Biotin"]
CultureMech_003153 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_003153 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003153 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_003153 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_003153 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_003153 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003153 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_003153 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
5.3243 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.20716 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.329138 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.145208 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.880929 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.193611 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
KBr
Br.K 119.002 g/mol
|
0.0222652 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.01151791 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.00551791, 0.006] |
| 9 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00880929 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.00474347 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.00116167 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
NaF
F.Na 41.988 g/mol
|
0.000648596 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 13 | HEPES | 2.30397 G_PER_L |
CHEBI:46756
HEPES
|
|
| 14 |
KNO3
K.NO3 101.102 g/mol
|
10 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 15 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
10 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 16 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 34 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 35 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 36 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix ingredients and adjust pH to 7.3. Fill the medium in a glass bottle, bubble with N2 for 30 min, then, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.658263Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J808 |
| 2026-03-13T03:08:50.914754Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:18.173554Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003153 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.993783+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFURIMONAS GD1 MEDIUM" to "sulfurimonas_gd1_medium" for consistent matching |
| 2026-06-05T07:41:47.734336+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.874226+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:12.132308+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |