Overview
- External ontology
- mediadive.medium:J809 (source link unavailable) — SPIROCHAETA STENOSTREPTA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=809
Composition
flowchart LR
CultureMech_003154["spirochaeta_stenostrepta_medium"]
ing0_Glucose["Glucose"]
CultureMech_003154 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_003154 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_003154 --> ing2_Peptone
ing3_Resazurin["Resazurin"]
CultureMech_003154 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Thioglycolate["Thioglycolate"]
CultureMech_003154 --> ing4_Thioglycolate
CHEBI_30066["CHEBI:30066"]
ing4_Thioglycolate -.-> CHEBI_30066
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 | Peptone | 2 G_PER_L | ||
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Thioglycolate
C2H3O2S 91.111 g/mol
|
0.5 G_PER_L |
CHEBI:30066
thioglycolate(1-)
|
Preparation
- Adjust pH to 7.6 with KOH before autoclaving. Add 1% agar for stabs. Pre-reduced medium is prepared under 100% nitrogen atmosphere.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.663676Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J809 |
| 2026-03-13T06:19:18.180880Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003154 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.002828+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA STENOSTREPTA MEDIUM" to "spirochaeta_stenostrepta_medium" for consistent matching |
| 2026-06-05T07:41:46.389050+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:52:40.011908+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |