Overview
- External ontology
- mediadive.medium:J819 (source link unavailable) — BACILLUS MACYAE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=819
Composition
flowchart LR
CultureMech_003163["bacillus_macyae_medium"]
ing0_NaCl["NaCl"]
CultureMech_003163 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_003163 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_NH4Cl["NH4Cl"]
CultureMech_003163 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_K2HPO4["K2HPO4"]
CultureMech_003163 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na2SO4["Na2SO4"]
CultureMech_003163 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003163 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003163 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NaHCO3["NaHCO3"]
CultureMech_003163 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Resazurin["Resazurin"]
CultureMech_003163 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Sodium_lactate["Sodium lactate"]
CultureMech_003163 --> ing9_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Sodium_lactate -.-> CHEBI_75228
ing10_Yeast_extract["Yeast extract"]
CultureMech_003163 --> ing10_Yeast_extract
ing11_KNO3["KNO3"]
CultureMech_003163 --> ing11_KNO3
CHEBI_63043["CHEBI:63043"]
ing11_KNO3 -.-> CHEBI_63043
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003163 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Biotin["Biotin"]
CultureMech_003163 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003163 --> ing14_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_pantothenate -.-> CHEBI_31345
ing15_HCl["HCl"]
CultureMech_003163 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_ZnCl2["ZnCl2"]
CultureMech_003163 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003163 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_003163 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003163 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003163 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003163 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003163 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Folic_acid["Folic acid"]
CultureMech_003163 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_003163 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_003163 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Lipoic_acid["Lipoic acid"]
CultureMech_003163 --> ing26_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1.18577 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.296443 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.296443 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.197628 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.296443 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.395257 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.148221 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.592885 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000494071 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
1.08696 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 11 | Yeast extract | 0.790514 G_PER_L | ||
| 12 |
KNO3
K.NO3 101.102 g/mol
|
0.494071 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.085 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
[Merged 2 duplicates: 0.08, 0.005] |
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.022 G_PER_L |
CHEBI:15956
biotin
|
[Merged 2 duplicates: 0.02, 0.002] |
| 15 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.10500000000000001 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
[Merged 2 duplicates: 0.1, 0.005] |
| 16 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Prepare the medium anaerobically under 100% N2. Add vitamins (filter-sterilized), sodium lactate and nitrate from sterile, anaerobic stock solutions. Adjust final pH to 7.4 - 7.8.
- Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Solutions
Trace element solution SL-10
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.5 G_PER_L |
Vitamin solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | [Merged 2 duplicates: 0.2, 0.005] |
| Pyridoxine hydrochloride | 0.3 G_PER_L | [Merged 2 duplicates: 0.3, 0.01] |
| Vitamin B12 | 0.1 G_PER_L | [Merged 2 duplicates: 0.1, 0.0001] |
Trace vitamins
Dose: 10 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nicotinic acid | 0.005 G_PER_L | [Merged 2 duplicates: 0.2, 0.005] |
| Pyridoxine hydrochloride | 0.01 G_PER_L | [Merged 2 duplicates: 0.3, 0.01] |
| Vitamin B12 | 0.0001 G_PER_L | [Merged 2 duplicates: 0.1, 0.0001] |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.688157Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J819 |
| 2026-03-13T03:09:35.810175Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:18.243989Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003163 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.074010+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACILLUS MACYAE MEDIUM" to "bacillus_macyae_medium" for consistent matching |
| 2026-06-05T07:40:50.283604+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.569149+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:53.719011+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-13T23:31:54.312806+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 1 -> 'Vitamin solution' @ 1 ml/l; 0 -> 'Trace vitamins' @ 10 ml/l. 3 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |