koko_medium

CultureMech:003169 bacterial COMPLEX LIQUID
Original name: KOKO MEDIUM

Overview

External ontology
mediadive.medium:J825 (source link unavailable) — KOKO MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.0}
Notes
JCM Medium 825 / KOKO Medium lists 930.0 ml/L distilled water, 0.16 g/L MgSO4 x 7 H2O, 1.0 g/L yeast extract, 0.06 g/L CaCl2 x 2 H2O, 0.5 g/L NH4Cl, 1.6 g/L K2HPO4, 0.5 mg/L resazurin, 1.0 g/L NaH2PO4 x 2 H2O, 1.0 g/L BD-Difco tryptone, 1.0 g/L peptone, 10.0 ml/L SL-4 trace element solution, 10.0 ml/L Trace vitamins, N2 gas, and sterile anaerobic 8% NaHCO3, 10% glucose, 3% Na2S x 9 H2O, and 3% L-cysteine HCl x H2O stocks; MediaDive J825 reports the same recipe at pH 7.0.

Composition

flowchart LR
    CultureMech_003169["koko_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003169 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003169 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003169 --> ing2_Yeast_extract
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003169 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_003169 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003169 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_003169 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_003169 --> ing7_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing7_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing8_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_003169 --> ing8_Tryptone__BD_Difco_
    ing9_Peptone["Peptone"]
    CultureMech_003169 --> ing9_Peptone
    ing10_N2["N2"]
    CultureMech_003169 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
930.0 ML_PER_L CHEBI:15377
water
 MediaDive J825 / JCM Medium 825 lists 930.0 ml/L Distilled water.
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.16 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 MediaDive J825 / JCM Medium 825 lists 0.16 g/L MgSO4 x 7 H2O.
3 Yeast extract 1.0 G_PER_L FOODON:03315426
Yeast extract
 MediaDive J825 / JCM Medium 825 lists 1.0 g/L Yeast extract.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.06 G_PER_L CHEBI:86158
calcium chloride dihydrate
 MediaDive J825 / JCM Medium 825 lists 0.06 g/L CaCl2 x 2 H2O.
5 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 MediaDive J825 / JCM Medium 825 lists 0.5 g/L NH4Cl.
6 K2HPO4
HO4P.2K 174.174 g/mol
1.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 MediaDive J825 / JCM Medium 825 lists 1.6 g/L K2HPO4.
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
 MediaDive J825 / JCM Medium 825 lists 0.5 mg/L Resazurin.
8 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
1.0 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
 MediaDive J825 / JCM Medium 825 lists 1.0 g/L NaH2PO4 x 2 H2O.
9 Tryptone (BD-Difco) 1.0 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 MediaDive J825 / JCM Medium 825 lists 1.0 g/L Tryptone (BD-Difco).
10 Peptone 1.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 MediaDive J825 / JCM Medium 825 lists 1.0 g/L Peptone.
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 MediaDive J825 / JCM Medium 825 lists N2 as an atmosphere component with no fixed liquid-phase concentration.

Preparation

  1. Mix the basal salts, yeast extract, BD-Difco tryptone, peptone, resazurin, 10.0 ml/L Trace element solution SL-4, 10.0 ml/L Trace vitamins, and 930.0 ml/L distilled water.
  2. Adjust the medium to pH 7.0.
  3. Autoclave the medium under an N2 atmosphere.
  4. After autoclaving, add 1.25 ml/L sterile anaerobic 8% NaHCO3 solution, 50.0 ml/L 10% glucose solution, 10.0 ml/L 3% Na2S x 9 H2O solution, and 10.0 ml/L 3% L-cysteine HCl x H2O solution.

Solutions

Trace element solution SL-4

Dose: 10.0 ML_PER_L

CultureMech:013111

Stock composition
IngredientConcentrationNotes
EDTA 0.5 G_PER_L MediaDive J825 / JCM Medium 825 lists 0.5 g/L EDTA.
FeSO4 x 7 H2O 0.2 G_PER_L MediaDive J825 / JCM Medium 825 lists 0.2 g/L FeSO4 x 7 H2O.
ZnSO4 x 7 H2O 0.01 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.1 g/L ZnSO4 x 7 H2O in SL-6 contributes 0.01 g/L in SL-4.
MnCl2 x 4 H2O 0.003 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.03 g/L MnCl2 x 4 H2O in SL-6 contributes 0.003 g/L in SL-4.
H3BO3 0.03 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.3 g/L H3BO3 in SL-6 contributes 0.03 g/L in SL-4.
CoCl2 x 6 H2O 0.02 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.2 g/L CoCl2 x 6 H2O in SL-6 contributes 0.02 g/L in SL-4.
CuCl2 x 2 H2O 0.001 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.01 g/L CuCl2 x 2 H2O in SL-6 contributes 0.001 g/L in SL-4.
NiCl2 x 6 H2O 0.002 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.02 g/L NiCl2 x 6 H2O in SL-6 contributes 0.002 g/L in SL-4.
Na2MoO4 x 2 H2O 0.003 G_PER_L MediaDive J825 / JCM Medium 825 expands 100.0 ml/L Trace element solution SL-6 into Trace element solution SL-4; 0.03 g/L Na2MoO4 x 2 H2O in SL-6 contributes 0.003 g/L in SL-4.
Distilled water 900.0 ML_PER_L MediaDive J825 / JCM Medium 825 lists 900.0 ml/L direct distilled water before adding 100.0 ml/L Trace element solution SL-6.
MediaDive J825 / JCM Medium 825 adds 10.0 ml/L Trace element solution SL-4. The inline stock composition expands the SL-6 trace-element stock that SL-4 contains.

Trace vitamins

Dose: 10.0 ML_PER_L

CultureMech:012935

Stock composition
IngredientConcentrationNotes
Biotin 2.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 2.0 mg/L Biotin.
Folic acid 2.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 2.0 mg/L Folic acid.
Pyridoxine hydrochloride 10.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 10.0 mg/L Pyridoxine hydrochloride.
Thiamine HCl 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L Thiamine HCl.
Riboflavin 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L Riboflavin.
Nicotinic acid 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L Nicotinic acid.
Calcium pantothenate 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L Calcium pantothenate.
Vitamin B12 0.1 MG_PER_L MediaDive J825 / JCM Medium 825 lists 0.1 mg/L Vitamin B12.
p-Aminobenzoic acid 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L p-Aminobenzoic acid.
Lipoic acid 5.0 MG_PER_L MediaDive J825 / JCM Medium 825 lists 5.0 mg/L Lipoic acid.
Distilled water 1000.0 ML_PER_L MediaDive J825 / JCM Medium 825 lists 1000.0 ml/L Distilled water.
MediaDive J825 / JCM Medium 825 adds 10.0 ml/L Trace vitamins from JCM Medium 197.

8% NaHCO3 solution

Dose: 1.25 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaHCO3 80.0 G_PER_L MediaDive J825 / JCM Medium 825 lists 80.0 g/L NaHCO3.
MediaDive J825 / JCM Medium 825 adds 1.25 ml/L sterile anaerobic 8% NaHCO3 stock.

10% Glucose solution

Dose: 50.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Glucose 100.0 G_PER_L MediaDive J825 / JCM Medium 825 lists 100.0 g/L Glucose.
MediaDive J825 / JCM Medium 825 adds 50.0 ml/L sterile anaerobic 10% glucose stock.

3% Na2S x 9 H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9 H2O 30.0 G_PER_L MediaDive J825 / JCM Medium 825 lists 30.0 g/L Na2S x 9 H2O.
MediaDive J825 / JCM Medium 825 adds 10.0 ml/L sterile anaerobic 3% Na2S x 9 H2O stock.

3% L-Cysteine HCl x H2O solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl x H2O 30.0 G_PER_L MediaDive J825 / JCM Medium 825 lists 30.0 g/L L-Cysteine HCl x H2O.
MediaDive J825 / JCM Medium 825 adds 10.0 ml/L sterile anaerobic 3% L-cysteine HCl x H2O stock.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.713011Z mediadive-import Imported from MediaDive Source: JCM, ID: J825
2026-03-13T06:19:18.304045Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003169
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.141556+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KOKO MEDIUM" to "koko_medium" for consistent matching
2026-06-05T07:41:10.479368+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.397157+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m860_koko_score15.py RESOLVED_JCM_825_KOKO_MEDIUM Replaced the previously flattened stock constituents with the source-level KOKO Medium recipe, corrected the water and resazurin units, restored the SL-4, Trace vitamins, glucose, NaHCO3, Na2S, and L-cysteine stock additions, and linked the TOGO M860 source duplicate.