modified_nms_medium

CultureMech:003172 bacterial DEFINED LIQUID
Original name: MODIFIED NMS MEDIUM

Overview

External ontology
mediadive.medium:J828 (source link unavailable) — MODIFIED NMS MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=828

Composition

flowchart LR
    CultureMech_003172["modified_nms_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003172 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003172 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_KNO3["KNO3"]
    CultureMech_003172 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003172 --> ing3_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing3_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_003172 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_KH2PO4["KH2PO4"]
    CultureMech_003172 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_003172 --> ing6_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing7_EDTA["EDTA"]
    CultureMech_003172 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003172 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003172 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003172 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_003172 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003172 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003172 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003172 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003172 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.985222 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.197044 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 KNO3
K.NO3 101.102 g/mol
0.985222 G_PER_L CHEBI:63043
potassium nitrate
4 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.27 G_PER_L CHEBI:86254
iron trichloride hexahydrate
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.372 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 KH2PO4
H2O4P.K 136.084 g/mol
2.72 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
7.17 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
8 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
  2. Adjust pH to 6.8, if necessary. Aseptically distribute the medium into sterile serum bottles (leave &; 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.727267Z mediadive-import Imported from MediaDive Source: JCM, ID: J828
2026-03-13T06:19:18.340076Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003172
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.182109+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED NMS MEDIUM" to "modified_nms_medium" for consistent matching
2026-06-05T07:41:37.526584+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.644774+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.884838+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.