Overview
- External ontology
- mediadive.medium:J832 (source link unavailable) — R2A AGAR WITH ARTIFICIAL SEAWATER
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 832 combines 18.2 g R2A agar (BD-Difco) with 1.0 L artificial seawater; the artificial seawater salts are printed in JCM Medium 736.
Composition
flowchart LR
CultureMech_003176["r2a_agar_with_artificial_seawater"]
ing0_R2A_agar__BD_Difco_["R2A agar (BD-Difco)"]
CultureMech_003176 --> ing0_R2A_agar__BD_Difco_
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | R2A agar (BD-Difco) | 18.2 G_PER_L | JCM Medium 832 lists 18.2 g R2A agar (BD-Difco) per liter of artificial seawater. |
Preparation
- Prepare artificial seawater from the JCM Medium 736 salts.
- Dissolve 18.2 g R2A agar (BD-Difco) in 1.0 L artificial seawater.
- Autoclave at 121 C for 15 min.
Solutions
Artificial seawater
Dose: 1000 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 23.5 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| MgCl2 x 6 H2O | 4.9 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| Na2SO4 | 3.9 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| CaCl2 x 2 H2O | 1.1 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| KCl | 0.66 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| NaHCO3 | 0.19 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| KBr | 0.096 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| H3BO3 | 0.026 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| SrCl2 x 6 H2O | 0.024 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| NaF | 0.003 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| Distilled water | 1000 ML_PER_L | JCM Medium 736 lists 1.0 L distilled water for the Artificial seawater subrecipe. |
JCM Medium 832 adds 1.0 L artificial seawater per liter of R2A Agar With Artificial Seawater; the salt composition is printed in JCM Medium 736.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.740833Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J832 |
| 2026-03-13T06:19:18.374442Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003176 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.221111+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A AGAR WITH ARTIFICIAL SEAWATER" to "r2a_agar_with_artificial_seawater" for consistent matching |
| 2026-06-05T07:41:43.585999+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.765927+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | RESOLVED_R2A_SCORE40_GRAPH | JCM Medium 832 combines 18.2 g R2A agar (BD-Difco) with 1.0 L artificial seawater; the artificial seawater salts are printed in JCM Medium 736. |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | LINKED_R2A_SCORE40_CHILDREN | Added or refreshed bacterial/TOGO_M868_R2A_Agar_With_Artificial_Seawater.yaml as a child of bacterial/r2a_agar_with_artificial_seawater.yaml. |