lind_bht_medium

CultureMech:003188 bacterial COMPLEX LIQUID
Original name: LIND BHT MEDIUM

Overview

External ontology
mediadive.medium:J843 (source link unavailable) — LIND BHT MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=843

Composition

flowchart LR
    CultureMech_003188["lind_bht_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003188 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003188 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003188 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003188 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003188 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003188 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_003188 --> ing6_Yeast_extract
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_003188 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003188 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Resazurin["Resazurin"]
    CultureMech_003188 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_NaHCO3["NaHCO3"]
    CultureMech_003188 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003188 --> ing11_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing11_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing12_Sodium_acetate["Sodium acetate"]
    CultureMech_003188 --> ing12_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing12_Sodium_acetate -.-> CHEBI_32954
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003188 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_HCl["HCl"]
    CultureMech_003188 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003188 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_H3BO3["H3BO3"]
    CultureMech_003188 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003188 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003188 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003188 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003188 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003188 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003188 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.297324 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.297324 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.99108 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
19.8216 G_PER_L CHEBI:26710
sodium chloride
5 KCl
Cl.K 74.551 g/mol
0.099108 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.099108 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Yeast extract 1.98216 G_PER_L
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
4.91576 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49554 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Resazurin
C12H7NO4 229.191 g/mol
0.00099108 G_PER_L CHEBI:8806
Resazurin
11 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
30 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
13 Sodium acetate
C2H3O2.Na 82.034 g/mol
20 G_PER_L CHEBI:32954
sodium acetate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 HCl
HCl 36.461 g/mol
12.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
  2. Then, aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere):
  3. Pressurize inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.767321Z mediadive-import Imported from MediaDive Source: JCM, ID: J843
2026-03-13T06:19:18.439525Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003188
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.293546+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LIND BHT MEDIUM" to "lind_bht_medium" for consistent matching
2026-06-05T07:41:11.200587+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.559452+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.