Overview
- External ontology
- mediadive.medium:J845 (source link unavailable) — 1/2 R2A AGAR
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 845 lists 9.1 g/L R2A agar (BD-Difco), 7.5 g/L agar, and 1.0 L distilled water.
Composition
flowchart LR
CultureMech_003190["1_2_r2a_agar"]
ing0_R2A_agar__BD_Difco_["R2A agar (BD-Difco)"]
CultureMech_003190 --> ing0_R2A_agar__BD_Difco_
ing1_Agar["Agar"]
CultureMech_003190 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Distilled_water["Distilled water"]
CultureMech_003190 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | R2A agar (BD-Difco) | 9.1 G_PER_L | JCM Medium 845 lists 9.1 g/L R2A agar (BD-Difco). | |
| 2 | Agar | 7.5 G_PER_L |
CHEBI:2509
agar
|
JCM Medium 845 adds 7.5 g/L agar. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 845 lists 1.0 L distilled water. |
Preparation
- Dissolve 9.1 g R2A agar (BD-Difco) and 7.5 g agar in 1.0 L distilled water.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.775292Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J845 |
| 2026-03-13T06:19:18.455598Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003190 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.324043+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/2 R2A AGAR" to "1_2_r2a_agar" for consistent matching |
| 2026-06-05T07:40:15.512325+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-08T00:00:00-07:00 | repair_jcm_r2a_score20.py | RESOLVED_JCM_R2A_SCORE20 | Uses 9.1 g/L R2A agar (BD-Difco) with 7.5 g/L added agar. |