gam_semisolid

CultureMech:003195 bacterial COMPLEX SEMISOLID
Original name: GAM SEMISOLID

Overview

External ontology
mediadive.medium:J84 (source link unavailable) — GAM SEMISOLID
Applications
  • Microbial cultivation
Notes
JCM Medium 84 contains 59.0 g GAM broth (Nissui), 2.0 g agar, and 1.0 L distilled water.

Composition

flowchart LR
    CultureMech_003195["gam_semisolid"]
    ing0_GAM_broth__Nissui_["GAM broth (Nissui)"]
    CultureMech_003195 --> ing0_GAM_broth__Nissui_
    ing1_Agar["Agar"]
    CultureMech_003195 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Distilled_water["Distilled water"]
    CultureMech_003195 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 GAM broth (Nissui) 59.0 G_PER_L JCM Medium 84 lists 59.0 g GAM broth (Nissui).
2 Agar 2.0 G_PER_L CHEBI:2509
agar
JCM Medium 84 lists 2.0 g agar.
3 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
JCM Medium 84 lists 1000 ml distilled water.

Preparation

  1. Suspend 59.0 g GAM broth (Nissui) and 2.0 g agar in 1.0 L distilled water.
  2. Autoclave at 121 C for 15 min unless otherwise stated.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.701848Z mediadive-import Imported from MediaDive Source: JCM, ID: J84
2026-03-13T06:19:18.497091Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003195
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.371505+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GAM SEMISOLID" to "gam_semisolid" for consistent matching
2026-06-05T07:41:05.942293+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_official_simple_score20.py RESOLVED_OFFICIAL_SIMPLE_SCORE20 JCM Medium 84 contains 59.0 g GAM broth (Nissui), 2.0 g agar, and 1.0 L distilled water.