Overview
- External ontology
- mediadive.medium:J851 (source link unavailable) — m TGE-GP MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 851 prepares m TGE-GP Medium by supplementing JCM Medium 795 with 5.0 g/L final glycerol and 0.1 mM final KH2PO4.
Composition
flowchart LR
CultureMech_003197["m_tge_gp_medium"]
ing0_Bacto_m_TGE_broth__BD_Difco_["Bacto m TGE broth (BD-Difco)"]
CultureMech_003197 --> ing0_Bacto_m_TGE_broth__BD_Difco_
ing1_Distilled_water["Distilled water"]
CultureMech_003197 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Glycerol["Glycerol"]
CultureMech_003197 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_KH2PO4["KH2PO4"]
CultureMech_003197 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto m TGE broth (BD-Difco) | 18.0 G_PER_L | JCM Medium 795 lists this commercial broth powder. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 795 lists 1.0 L distilled water. |
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
5.0 G_PER_L |
CHEBI:17754
glycerol
|
JCM Medium 851 supplements JCM Medium 795 with 5.0 g/L final glycerol. |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 851 supplements JCM Medium 795 with 0.1 mM final KH2PO4. |
Preparation
- Use JCM Medium 795 with 5.0 g/L final glycerol and 0.1 mM final KH2PO4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.797098Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J851 |
| 2026-02-03T11:26:56.353511 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:18.512942Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003197 |
| 2026-03-17T19:45:32.389437+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "m TGE-GP MEDIUM" to "m_tge_gp_medium" for consistent matching |
| 2026-04-04T18:49:20.059895 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from m_tge_broth_medium (CultureMech:003139) |
| 2026-05-16T08:57:37.823850+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-09-06T00:00:00-07:00 | repair_jcm_commercial_broths_score40.py | RESOLVED_JCM_COMMERCIAL_BROTH_SCORE40_GRAPH | JCM Medium 851 prepares m TGE-GP Medium by supplementing JCM Medium 795 with 5.0 g/L final glycerol and 0.1 mM final KH2PO4. |