Overview
- External ontology
- mediadive.medium:J853 (source link unavailable) — MRS FRUCTOSE MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 853 contains 55.0 g Lactobacilli MRS broth (BD-Difco), 10.0 g fructose, and 1.0 L distilled water.
Composition
flowchart LR
CultureMech_003199["mrs_fructose_medium"]
ing0_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
CultureMech_003199 --> ing0_Lactobacilli_MRS_broth__BD_Difco_
ing1_Fructose["Fructose"]
CultureMech_003199 --> ing1_Fructose
CHEBI_28757["CHEBI:28757"]
ing1_Fructose -.-> CHEBI_28757
ing2_Distilled_water["Distilled water"]
CultureMech_003199 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Lactobacilli MRS broth (BD-Difco) | 55.0 G_PER_L | JCM Medium 853 lists 55.0 g Lactobacilli MRS broth (BD-Difco). | |
| 2 | Fructose | 10.0 G_PER_L |
CHEBI:28757
fructose
|
JCM Medium 853 lists 10.0 g fructose. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 853 lists 1000 ml distilled water. |
Preparation
- Suspend 55.0 g Lactobacilli MRS broth (BD-Difco) and 10.0 g fructose in 1.0 L distilled water.
- Autoclave at 121 C for 15 min unless otherwise stated.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.803664Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J853 |
| 2026-03-13T06:19:18.529679Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003199 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.408305+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS FRUCTOSE MEDIUM" to "mrs_fructose_medium" for consistent matching |
| 2026-06-05T07:41:38.474451+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_official_simple_score20.py | RESOLVED_OFFICIAL_SIMPLE_SCORE20 | JCM Medium 853 contains 55.0 g Lactobacilli MRS broth (BD-Difco), 10.0 g fructose, and 1.0 L distilled water. |