colon_extract_medium

CultureMech:003200 bacterial COMPLEX LIQUID
Original name: COLON EXTRACT MEDIUM

Overview

External ontology
mediadive.medium:J854 (source link unavailable) — COLON EXTRACT MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=854

Composition

flowchart LR
    CultureMech_003200["colon_extract_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003200 --> ing0_Yeast_extract
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003200 --> ing1_Trypticase_peptone
    ing2_NaHCO3["NaHCO3"]
    CultureMech_003200 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Resazurin["Resazurin"]
    CultureMech_003200 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003200 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Vitamin_K1["Vitamin K1"]
    CultureMech_003200 --> ing5_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing5_Vitamin_K1 -.-> CHEBI_18067
    ing6_NaCl["NaCl"]
    CultureMech_003200 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_KH2PO4["KH2PO4"]
    CultureMech_003200 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003200 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003200 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003200 --> ing10_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003200 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003200 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003200 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_K2HPO4["K2HPO4"]
    CultureMech_003200 --> ing14_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_Glucose["Glucose"]
    CultureMech_003200 --> ing15_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing15_Glucose -.-> CHEBI_17234
    ing16_Maltose["Maltose"]
    CultureMech_003200 --> ing16_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing16_Maltose -.-> CHEBI_17306
    ing17_Cellobiose["Cellobiose"]
    CultureMech_003200 --> ing17_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing17_Cellobiose -.-> CHEBI_17057
    ing18_Xylose["Xylose"]
    CultureMech_003200 --> ing18_Xylose
    CHEBI_18222["CHEBI:18222"]
    ing18_Xylose -.-> CHEBI_18222

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Yeast extract 3.15657 G_PER_L
2 Trypticase peptone 3.15657 G_PER_L
3 NaHCO3
CHO3.Na 84.006 g/mol
3.15657 G_PER_L CHEBI:32139
sodium hydrogencarbonate
4 Resazurin
C12H7NO4 229.191 g/mol
0.000631313 G_PER_L CHEBI:8806
Resazurin
5 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.631313 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
6 Vitamin K1
C31H46O2 450.707 g/mol
0.2 G_PER_L CHEBI:18067
phylloquinone
7 NaCl
Cl.Na 58.443 g/mol
5.4 G_PER_L CHEBI:26710
sodium chloride
8 KH2PO4
H2O4P.K 136.084 g/mol
2.7 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.16 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.12 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.07 G_PER_L CHEBI:86360
manganese(II) sulfate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.06 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 (NH4)2SO4
2H4N.O4S 132.141 g/mol
5.4 G_PER_L CHEBI:62946
ammonium sulfate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.05 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 K2HPO4
HO4P.2K 174.174 g/mol
2.7 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
16 Glucose
C6H12O6 180.156 g/mol
50 G_PER_L CHEBI:17234
glucose
17 Maltose
C12H22O11 342.297 g/mol
50 G_PER_L CHEBI:17306
maltose
18 Cellobiose
C12H22O11 342.297 g/mol
50 G_PER_L CHEBI:17057
cellobiose
19 Xylose
C5H10O5 150.13 g/mol
50 G_PER_L CHEBI:18222
xylose

Preparation

  1. Adjust pH to 7.0.
  2. Dilute (1:1, w/v) the content of terminal pig colon with the following buffer: 167 ml Solution A (see above) and 167 ml Solution B (see above). Make up to 1.0 L with distilled water. Stomach the suspension and filter through a cheese cloth. Incubate at 37C for 48 h and adjust pH to 7.0. Centrifuge at 20,000 x g for 20 min. Retrieve the supernatant and store at -20C until use.
  3. Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.805467Z mediadive-import Imported from MediaDive Source: JCM, ID: J854
2026-03-13T06:19:18.536418Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003200
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.415475+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "COLON EXTRACT MEDIUM" to "colon_extract_medium" for consistent matching
2026-06-05T07:40:54.622139+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.939583+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.