25_glucose_medium

CultureMech:003216 bacterial COMPLEX SOLID_AGAR
Original name: 25% GLUCOSE MEDIUM

Overview

External ontology
mediadive.medium:J86 (source link unavailable) — 25% GLUCOSE MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=86

Composition

flowchart LR
    CultureMech_003216["25_glucose_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_003216 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Polypepton__Nihon_Pharm__Co__["Polypepton (Nihon Pharm. Co.)"]
    CultureMech_003216 --> ing1_Polypepton__Nihon_Pharm__Co__
    ing2_Malt_extract["Malt extract"]
    CultureMech_003216 --> ing2_Malt_extract
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_003216 --> ing3_Yeast_extract
    ing4_Agar["Agar"]
    CultureMech_003216 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Distilled_water["Distilled water"]
    CultureMech_003216 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
250 G_PER_L CHEBI:17234
glucose
JCM Medium 86 lists 250 g glucose.
2 Polypepton (Nihon Pharm. Co.) 5 G_PER_L JCM Medium 86 lists the Nihon Pharmaceutical Polypepton product.
3 Malt extract 3 G_PER_L JCM Medium 86 lists this generic malt extract.
4 Yeast extract 3 G_PER_L FOODON:03315426
yeast extract
JCM Medium 86 lists this generic yeast extract.
5 Agar 25 G_PER_L CHEBI:2509
agar
JCM Medium 86 lists 25 g agar.
6 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
JCM Medium 86 lists 1.0 L distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.705138Z mediadive-import Imported from MediaDive Source: JCM, ID: J86
2026-03-13T06:19:18.638319Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003216
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.529293+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "25% GLUCOSE MEDIUM" to "25_glucose_medium" for consistent matching
2026-06-05T07:40:15.706315+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_sparse_top_score15.py RESOLVED_SPARSE_TOP_SCORE15 JCM Medium 86 lists 250 g glucose, 5 g Polypepton, 3 g malt extract, 3 g yeast extract, 25 g agar, and 1 L distilled water.