proteiniphilus_5mh_medium

CultureMech:003218 bacterial COMPLEX LIQUID
Original name: PROTEINIPHILUS 5MH MEDIUM

Overview

External ontology
mediadive.medium:J871 (source link unavailable) — PROTEINIPHILUS 5MH MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=871

Composition

flowchart LR
    CultureMech_003218["proteiniphilus_5mh_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003218 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003218 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003218 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003218 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_003218 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_003218 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_003218 --> ing6_Yeast_extract
    ing7_NaHCO3["NaHCO3"]
    CultureMech_003218 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Resazurin["Resazurin"]
    CultureMech_003218 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003218 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003218 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003218 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003218 --> ing12_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing12_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003218 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003218 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_ZnCl2["ZnCl2"]
    CultureMech_003218 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003218 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_H3BO3["H3BO3"]
    CultureMech_003218 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003218 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003218 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003218 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003218 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.494071 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.494071 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1988142 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0988142, 0.1]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.296443 G_PER_L CHEBI:31206
ammonium chloride
5 KH2PO4
H2O4P.K 136.084 g/mol
0.197628 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 KCl
Cl.K 74.551 g/mol
0.494071 G_PER_L CHEBI:32588
potassium chloride
7 Yeast extract 1.97628 G_PER_L
8 NaHCO3
CHO3.Na 84.006 g/mol
2.47036 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Resazurin
C12H7NO4 229.191 g/mol
0.000988142 G_PER_L CHEBI:8806
Resazurin
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.004 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components except NaHCO3, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. While gassing, add NaHCO3 to the medium, distribute it into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, stand the medium overnight. Prior to inoculation, add per liter the following solutions (autoclaved under a N2 atmosphere):
  2. Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.852570Z mediadive-import Imported from MediaDive Source: JCM, ID: J871
2026-03-13T03:09:28.045220Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:18.654484Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003218
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.541542+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PROTEINIPHILUS 5MH MEDIUM" to "proteiniphilus_5mh_medium" for consistent matching
2026-06-05T07:41:42.584311+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.422473+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.