methanobacterium_medium_iv

Overview

External ontology

mediadive.medium:J872 (source link unavailable) — METHANOBACTERIUM MEDIUM (IV)

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=872 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_003219["methanobacterium_medium_iv"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003219 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003219 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_003219 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003219 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003219 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003219 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_003219 --> ing6_Yeast_extract
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_003219 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Sodium_formate["Sodium formate"]
    CultureMech_003219 --> ing8_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing8_Sodium_formate -.-> CHEBI_62965
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003219 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Resazurin["Resazurin"]
    CultureMech_003219 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003219 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003219 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Sludge["Sludge"]
    CultureMech_003219 --> ing13_Sludge
    ing14_HCl["HCl"]
    CultureMech_003219 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003219 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_003219 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003219 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_003219 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003219 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003219 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003219 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003219 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_Valeric_acid["Valeric acid"]
    CultureMech_003219 --> ing23_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing23_Valeric_acid -.-> CHEBI_17418
    ing24_Isovaleric_acid["Isovaleric acid"]
    CultureMech_003219 --> ing24_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing24_Isovaleric_acid -.-> CHEBI_28484
    ing25_Tryptone["Tryptone"]
    CultureMech_003219 --> ing25_Tryptone
    ing26_Yeast_extract["Yeast extract"]
    CultureMech_003219 --> ing26_Yeast_extract
    ing27_Sodium_chloride["Sodium chloride"]
    CultureMech_003219 --> ing27_Sodium_chloride
    ing27_Sodium_chloride -.-> CHEBI_26710
    ing28_Isobutyric_acid["Isobutyric acid"]
    CultureMech_003219 --> ing28_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing28_Isobutyric_acid -.-> CHEBI_16135

Ingredients (29)

Preparation

1. Mix components except NaHCO3 and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
2. Pressurize inoculated vessels to 200 kPa with a H2-CO2 (80:20, v/v) gas mixture.
3. Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.
4. Adjust pH to 7.5 with concentrated NaOH solution.

Target organisms

No target organisms are recorded.

Provenance