phosphoac3_medium

CultureMech:003222 bacterial COMPLEX LIQUID
Original name: PHOSPHOAC3 MEDIUM

Overview

External ontology
mediadive.medium:J875 (source link unavailable) — PHOSPHOAC3 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=875

Composition

flowchart LR
    CultureMech_003222["phosphoac3_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003222 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003222 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003222 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003222 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003222 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003222 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003222 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_003222 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003222 --> ing8_Yeast_extract
    ing9_Sulfur["Sulfur"]
    CultureMech_003222 --> ing9_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing9_Sulfur -.-> CHEBI_26833
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003222 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Resazurin["Resazurin"]
    CultureMech_003222 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_NaHCO3["NaHCO3"]
    CultureMech_003222 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_L_Sodium_lactate["L-Sodium lactate"]
    CultureMech_003222 --> ing13_L_Sodium_lactate
    CHEBI_232798["CHEBI:232798"]
    ing13_L_Sodium_lactate -.-> CHEBI_232798
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003222 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003222 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003222 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003222 --> ing17_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003222 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003222 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003222 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003222 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003222 --> ing22_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing22_AlK_SO4_2 -.-> CHEBI_86463
    ing23_H3BO3["H3BO3"]
    CultureMech_003222 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003222 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.28222 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.28222 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.940734 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
2.88147 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1.88147, 1.0]
5 KCl
Cl.K 74.551 g/mol
0.0940734 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1940734 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0940734, 0.1]
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.470367 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.188147 G_PER_L CHEBI:32954
sodium acetate
9 Yeast extract 1.88147 G_PER_L
10 Sulfur
S 32.067 g/mol
9.40734 G_PER_L CHEBI:26833
sulfur atom
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.470367 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Resazurin
C12H7NO4 229.191 g/mol
0.000940734 G_PER_L CHEBI:8806
Resazurin
13 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 L-Sodium lactate
C3H5O3.Na 112.06 g/mol
20 G_PER_L CHEBI:232798
sodium L-lactate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
24 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur while gassing with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:17.867340Z mediadive-import Imported from MediaDive Source: JCM, ID: J875
2026-03-13T03:09:23.184184Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:18.692303Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003222
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.584462+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PHOSPHOAC3 MEDIUM" to "phosphoac3_medium" for consistent matching
2026-06-05T07:41:41.808069+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.202184+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.