phosphate_buffered_saline_ph_7_4

CultureMech:003233 bacterial DEFINED LIQUID
Original name: PHOSPHATE-BUFFERED SALINE (pH 7.4)

Overview

External ontology
mediadive.medium:J885 (source link unavailable) — PHOSPHATE-BUFFERED SALINE (pH 7.4)
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=885

Composition

flowchart LR
    CultureMech_003233["phosphate_buffered_saline_ph_7_4"]
    ing0_NaCl["NaCl"]
    CultureMech_003233 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_003233 --> ing1_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing2_KCl["KCl"]
    CultureMech_003233 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003233 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
8 G_PER_L CHEBI:26710
sodium chloride
2 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
2.9 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate

Preparation

  1. Unadjusted pH should be 7.4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.901013Z mediadive-import Imported from MediaDive Source: JCM, ID: J885
2026-03-13T06:19:18.804103Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003233
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.678079+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PHOSPHATE-BUFFERED SALINE (pH 7.4)" to "phosphate_buffered_saline_ph_7_4" for consistent matching
2026-06-05T07:41:41.796972+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.200364+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.