natronolimnobius_aht32_medium_a

CultureMech:003235 archaea COMPLEX LIQUID
Original name: NATRONOLIMNOBIUS AHT32 MEDIUM (A)

Overview

External ontology
mediadive.medium:J887 (source link unavailable) — NATRONOLIMNOBIUS AHT32 MEDIUM (A)
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=887

Composition

flowchart LR
    CultureMech_003235["natronolimnobius_aht32_medium_a"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_003235 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_003235 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_003235 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_003235 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003235 --> ing4_Trace_vitamins__see_Medium_No__197_
    ing5_NH4Cl["NH4Cl"]
    CultureMech_003235 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_MgSO4["MgSO4"]
    CultureMech_003235 --> ing6_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing6_MgSO4 -.-> CHEBI_32599
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_003235 --> ing7_Yeast_extract
    ing8_Sodium_butyrate["Sodium butyrate"]
    CultureMech_003235 --> ing8_Sodium_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing8_Sodium_butyrate -.-> CHEBI_64103
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_003235 --> ing9_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate -.-> CHEBI_32954
    ing10_EDTA["EDTA"]
    CultureMech_003235 --> ing10_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing10_EDTA -.-> CHEBI_4735
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003235 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003235 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003235 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_003235 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003235 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003235 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003235 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003235 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
3518.52 G_PER_L CHEBI:29377
sodium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
555.556 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NaCl
Cl.Na 58.443 g/mol
296.296 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
37.037 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Trace vitamins (see Medium No. 197) 10 G_PER_L
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
7 MgSO4
Mg.O4S 120.368 g/mol
1 G_PER_L CHEBI:32599
magnesium sulfate
8 Yeast extract 0.2 G_PER_L
9 Sodium butyrate
C4H7O2.Na 110.088 g/mol
20 G_PER_L CHEBI:64103
sodium butyrate
10 Sodium acetate
C2H3O2.Na 82.034 g/mol
20 G_PER_L CHEBI:32954
sodium acetate
11 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Then, aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
  2. Distribute the medium into culture vessels, replace the gas phase with N2 containing 5% O2 (by vol.) and close with butyl rubber stoppers.

Solutions

Selenite-tungstate solution (see Medium No. 431)

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.905528Z mediadive-import Imported from MediaDive Source: JCM, ID: J887
2026-03-09T08:05:01.357107+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:18.817644Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003235
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.694169+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOLIMNOBIUS AHT32 MEDIUM (A)" to "natronolimnobius_aht32_medium_a" for consistent matching
2026-05-16T09:04:27.400775+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:39.388931+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.447053+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.