Overview
- External ontology
- mediadive.medium:J889 (source link unavailable) — METALLIBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=889
Composition
flowchart LR
CultureMech_003237["metallibacterium_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_003237 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003237 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_003237 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003237 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_003237 --> ing4_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing4_VOSO4_x_n_H2O -.-> CHEBI_87020
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003237 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_003237 --> ing6_Trypticase_peptone
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003237 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003237 --> ing8_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing8_MnSO4_x_n_H2O -.-> CHEBI_86360
ing9_NaCl["NaCl"]
CultureMech_003237 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003237 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003237 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003237 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003237 --> ing13_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing14_H3BO3["H3BO3"]
CultureMech_003237 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003237 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_003237 --> ing16__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing16__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003237 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_Na2SeO4["Na2SeO4"]
CultureMech_003237 --> ing18_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing18_Na2SeO4 -.-> CHEBI_77775
ing19_Biotin["Biotin"]
CultureMech_003237 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_003237 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003237 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_003237 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_003237 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_003237 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003237 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_003237 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003237 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_003237 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.130564 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0524233 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0267062 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.1454010000000001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.145401, 1.0] |
| 5 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
0.00049456 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.0098912 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.0098912, 1.0] |
| 7 | Trypticase peptone | 1.38477 G_PER_L | ||
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components, except Trace vitamins, adjust pH to 5.5 and autoclave. After cooling, aseptically add filter-sterilized Trace vitamins solution.
- First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.911299Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J889 |
| 2026-03-13T03:09:15.043673Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:18.833262Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003237 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.711692+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METALLIBACTERIUM MEDIUM" to "metallibacterium_medium" for consistent matching |
| 2026-06-05T07:40:17.843206+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.115209+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:43.155311+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |