1_ogawa_medium

CultureMech:003238 bacterial COMPLEX LIQUID
Original name: 1% OGAWA MEDIUM

Overview

External ontology
mediadive.medium:J88 (source link unavailable) — 1% OGAWA MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=88

Composition

flowchart LR
    CultureMech_003238["1_ogawa_medium"]
    ing0_Na_glutamate["Na glutamate"]
    CultureMech_003238 --> ing0_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing0_Na_glutamate -.-> CHEBI_64220
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003238 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Glycerol["Glycerol"]
    CultureMech_003238 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_Malachite_green["Malachite green"]
    CultureMech_003238 --> ing3_Malachite_green
    CHEBI_72449["CHEBI:72449"]
    ing3_Malachite_green -.-> CHEBI_72449
    ing4_Whole_egg["Whole egg"]
    CultureMech_003238 --> ing4_Whole_egg

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Na glutamate
C5H8NO4.Na 169.112 g/mol
10 G_PER_L CHEBI:64220
monosodium glutamate
2 KH2PO4
H2O4P.K 136.084 g/mol
10 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Glycerol
C3H8O3 92.094 g/mol
6 G_PER_L CHEBI:17754
glycerol
4 Malachite green
C23H25N2.Cl 364.92 g/mol
6 G_PER_L CHEBI:72449
malachite green
5 Whole egg 200 G_PER_L

Preparation

  1. Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
  2. Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.708737Z mediadive-import Imported from MediaDive Source: JCM, ID: J88
2026-03-13T06:19:18.839956Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003238
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.720165+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1% OGAWA MEDIUM" to "1_ogawa_medium" for consistent matching
2026-06-05T07:40:15.673987+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.517307+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.