Overview
- External ontology
- mediadive.medium:J904 (source link unavailable) — HALOBACTERIA STARCH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=904
Composition
flowchart LR
CultureMech_003253["halobacteria_starch_medium"]
ing0_Starch["Starch"]
CultureMech_003253 --> ing0_Starch
CHEBI_28017["CHEBI:28017"]
ing0_Starch -.-> CHEBI_28017
ing1_Sodium_glutamate_monohydrate["Sodium glutamate monohydrate"]
CultureMech_003253 --> ing1_Sodium_glutamate_monohydrate
CHEBI_232425["CHEBI:232425"]
ing1_Sodium_glutamate_monohydrate -.-> CHEBI_232425
ing2_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003253 --> ing2_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing2_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003253 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KCl["KCl"]
CultureMech_003253 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_003253 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003253 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003253 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Starch | 10 G_PER_L |
CHEBI:28017
starch
|
|
| 2 |
Sodium glutamate monohydrate
C5H8NO4.H2O.Na 187.127 g/mol
|
1 G_PER_L |
CHEBI:232425
monosodium L-glutamate hydrate
|
|
| 3 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 MG_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 MG_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2.
- For preparation of solid medium, add 20.0 g/L agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.958324Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J904 |
| 2026-01-28T19:48:32.568265Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.949680Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003253 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.845095+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIA STARCH MEDIUM" to "halobacteria_starch_medium" for consistent matching |
| 2026-06-05T07:41:07.569572+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |