halorhodospira_neutriphila_medium

CultureMech:003257 bacterial COMPLEX LIQUID
Original name: HALORHODOSPIRA NEUTRIPHILA MEDIUM

Overview

External ontology
mediadive.medium:J908 (source link unavailable) — HALORHODOSPIRA NEUTRIPHILA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=908

Composition

flowchart LR
    CultureMech_003257["halorhodospira_neutriphila_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003257 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003257 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_003257 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003257 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003257 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003257 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NaHCO3["NaHCO3"]
    CultureMech_003257 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003257 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_Sodium_acetate["Sodium acetate"]
    CultureMech_003257 --> ing8_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Sodium_acetate -.-> CHEBI_32954
    ing9_Sodium_succinate["Sodium succinate"]
    CultureMech_003257 --> ing9_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing9_Sodium_succinate -.-> CHEBI_63675
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_003257 --> ing10_Yeast_extract
    ing11_Na2_EDTA["Na2-EDTA"]
    CultureMech_003257 --> ing11_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing11_Na2_EDTA -.-> CHEBI_64734
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003257 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003257 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003257 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_ZnCl2["ZnCl2"]
    CultureMech_003257 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003257 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003257 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_003257 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003257 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_003257 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003257 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_D_____biotin["D-(+)-biotin"]
    CultureMech_003257 --> ing22_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_D_____biotin -.-> CHEBI_15956
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003257 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003257 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003257 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_003257 --> ing26_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing26_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.956023 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.478011 G_PER_L CHEBI:31206
ammonium chloride
3 NaCl
Cl.Na 58.443 g/mol
95.6023 G_PER_L CHEBI:26710
sodium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1.91205 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.478011 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0478011 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
20 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
7.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 Sodium acetate
C2H3O2.Na 82.034 g/mol
4 G_PER_L CHEBI:32954
sodium acetate
10 Sodium succinate
C4H4O4.2Na 162.052 g/mol
2 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
11 Yeast extract 10 G_PER_L
12 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.05 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.1 G_PER_L CHEBI:15940
nicotinic acid
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.025 G_PER_L CHEBI:31345
Calcium pantothenate
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.25 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Adjust pH to 7.0 with 1 M Na2CO3 or 1 M H2SO4. Mix ingredients, except NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution, and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Separately autoclave acetate solution, succinate solution and yeast extract solution. Filter-sterilize NaHCO3, Na2S x 9H2O. Aseptically and anaerobically add NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution to the medium.
  2. Autoclave separately.
  3. Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.971627Z mediadive-import Imported from MediaDive Source: JCM, ID: J908
2026-03-13T06:19:18.988432Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003257
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.888246+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALORHODOSPIRA NEUTRIPHILA MEDIUM" to "halorhodospira_neutriphila_medium" for consistent matching
2026-06-05T07:41:08.152978+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.644433+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.