methanocella_conradii_medium (CultureMech:003260)

Overview

External ontology

mediadive.medium:J911 (source link unavailable) — METHANOCELLA CONRADII MEDIUM

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=911

Composition

flowchart LR
    CultureMech_003260["methanocella_conradii_medium"]
    ing0_KCl["KCl"]
    CultureMech_003260 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003260 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_003260 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003260 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003260 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_003260 --> ing5_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing5_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003260 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_003260 --> ing7_Yeast_extract
    ing8_Resazurin["Resazurin"]
    CultureMech_003260 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003260 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003260 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_NaOH["NaOH"]
    CultureMech_003260 --> ing11_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing11_NaOH -.-> CHEBI_32145
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003260 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_HCl["HCl"]
    CultureMech_003260 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003260 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_003260 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003260 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_003260 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003260 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003260 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003260 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003260 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003260 --> ing22_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing23_Biotin["Biotin"]
    CultureMech_003260 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003260 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003260 --> ing25_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003260 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Sodium_phosphate_buffer["Sodium phosphate buffer"]
    CultureMech_003260 --> ing27_Sodium_phosphate_buffer
    ing28_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003260 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_003260 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	KCl Cl.K 74.551 g/mol	0.480307 G_PER_L	CHEBI:32588 potassium chloride
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.384246 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
3	KH2PO4 H2O4P.K 136.084 g/mol	0.192123 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
4	NH4Cl Cl.H4N 53.492 g/mol	0.0960615 G_PER_L	CHEBI:31206 ammonium chloride
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.0960615 G_PER_L	CHEBI:86158 calcium chloride dihydrate
6	Sodium acetate x 3 H2O C2H3O2.Na 82.034 g/mol	0.0787704 G_PER_L	CHEBI:32954 sodium acetate
7	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.288184 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
8	Yeast extract	0.192123 G_PER_L
9	Resazurin C12H7NO4 229.191 g/mol	0.000480307 G_PER_L	CHEBI:8806 Resazurin
10	NaHCO3 CHO3.Na 84.006 g/mol	30 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
11	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate
12	NaOH HO.Na 39.997 g/mol	0.4 G_PER_L	CHEBI:32145 sodium hydroxide
13	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.007 G_PER_L	CHEBI:63939 sodium tungstate dihydrate
14	HCl HCl 36.461 g/mol	2.5 G_PER_L	CHEBI:17883 hydrogen chloride
15	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate
16	ZnCl2 2Cl.Zn 136.296 g/mol	0.07 G_PER_L	CHEBI:49976 zinc dichloride
17	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.1 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
18	H3BO3 H3BO3 61.833 g/mol	0.006 G_PER_L	CHEBI:33118 boric acid
19	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.19 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate
20	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.002 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate
21	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.024 G_PER_L	CHEBI:34887 nickel dichloride
22	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.036 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
23	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.04 G_PER_L	CHEBI:30753 4-aminobenzoic acid
24	Biotin C10H16N2O3S 244.316 g/mol	0.01 G_PER_L	CHEBI:15956 biotin
25	Nicotinic acid C6H5NO2 123.111 g/mol	0.1 G_PER_L	CHEBI:15940 nicotinic acid
26	DL-Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.05 G_PER_L	CHEBI:31345 Calcium pantothenate
27	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	0.1 G_PER_L	CHEBI:30961 pyridoxine hydrochloride
28	Sodium phosphate buffer	100.0 G_PER_L		[Collapsed 2 identical duplicates: 100.0, 100.0]
29	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.1 G_PER_L	CHEBI:49105 thiamine hydrochloride
30	Vitamin B12	0.05 G_PER_L	CHEBI:176843 vitamin B12

Preparation

Mix components thoroughly, bring to a boil and cool down under a stream of N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically, add per liter the following solutions (filter-sterilized):
Prior to inoculation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture and add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 gas atmosphere):
Pressurize the inoculate culture vessels to 150 kPa H2-CO2 (4:1, v/v).
Comment: For strain JCM 17849, inoculate with a 10% inoculum. When the cellular density increases, re-pressure the culture vessels to 150 kPa with the same gas mixture.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.982544Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J911
2026-03-13T03:09:35.311354Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:19.009895Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003260
2026-03-17T06:30:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.911572+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOCELLA CONRADII MEDIUM" to "methanocella_conradii_medium" for consistent matching
2026-06-05T07:40:17.895342+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.167933+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:07.899688+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.