r2a_agar_with_1_nacl

CultureMech:003261 bacterial COMPLEX SOLID_AGAR
Original name: R2A AGAR WITH 1% NaCl

Overview

External ontology
mediadive.medium:J912 (source link unavailable) — R2A AGAR WITH 1% NaCl
Applications
  • Microbial cultivation
Notes
JCM Medium 912 lists 18.2 g/L R2A agar (BD-Difco), 10 g/L NaCl, and 1.0 L distilled water.

Composition

flowchart LR
    CultureMech_003261["r2a_agar_with_1_nacl"]
    ing0_NaCl["NaCl"]
    CultureMech_003261 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
Adds 10 g/L NaCl to R2A Agar.

Preparation

  1. Adds 10 g/L NaCl to R2A Agar.
  2. Autoclave at 121 C for 15 min.

Solutions

R2A Agar

Dose: 1000 ML_PER_L

CultureMech:002706

Adds 10 g/L NaCl to R2A Agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.987199Z mediadive-import Imported from MediaDive Source: JCM, ID: J912
2026-03-13T06:19:19.016081Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003261
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.918995+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A AGAR WITH 1% NaCl" to "r2a_agar_with_1_nacl" for consistent matching
2026-06-05T07:41:43.574442+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-08T00:00:00-07:00 repair_jcm_r2a_score20.py RESOLVED_JCM_R2A_SCORE20 Adds 10 g/L NaCl to R2A Agar.