semi_solid_autotrophic_growth_medium_for_magnetococcus_marinus

Overview

External ontology

mediadive.medium:J915 (source link unavailable) — SEMI-SOLID AUTOTROPHIC GROWTH MEDIUM FOR MAGNETOCOCCUS MARINUS

Applications

• Microbial cultivation

pH

7.1

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=915

Composition

flowchart LR
    CultureMech_003263["semi_solid_autotrophic_growth_medium_for_magnetococcus_marinus"]
    ing0_NaCl["NaCl"]
    CultureMech_003263 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003263 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_Na2SO4["Na2SO4"]
    CultureMech_003263 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_KCl["KCl"]
    CultureMech_003263 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003263 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_003263 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_003263 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Agarose["Agarose"]
    CultureMech_003263 --> ing7_Agarose
    CHEBI_2511["CHEBI:2511"]
    ing7_Agarose -.-> CHEBI_2511
    ing8_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_003263 --> ing8_Potassium_phosphate_buffer
    ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_003263 --> ing9_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003263 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_NaHCO3["NaHCO3"]
    CultureMech_003263 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_FeSO4_x_5_H2O["FeSO4 x 5 H2O"]
    CultureMech_003263 --> ing12_FeSO4_x_5_H2O
    CHEBI_75832["CHEBI:75832"]
    ing12_FeSO4_x_5_H2O -.-> CHEBI_75832
    ing13_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003263 --> ing13_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_HCl -.-> CHEBI_49105
    ing14_Myo_inositol["Myo-inositol"]
    CultureMech_003263 --> ing14_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing14_Myo_inositol -.-> CHEBI_17268
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003263 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003263 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_003263 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843
    ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003263 --> ing18_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003263 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Biotin["Biotin"]
    CultureMech_003263 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_003263 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470

Ingredients (22)

Preparation

1. Adjust pH to 7.2.
2. Mix components thoroughly, except agar, and adjust pH to 7.0. Add agarose to the medium and autoclave under a N2 gas atmosphere. After cooling to 50C, add the following solutions to the medium aseptically and anaerobically (autoclaved or *filter-sterilized):
3. Keep the medium temperature at 50C until the medium is distributed into sterile screw-capped test tubes as described below.
4. Adjust pH to 7.0 - 7.2 with sterile HCl or NaOH solution. Then, aseptically and anaerobically, add the following solution (autoclaved):
5. Dispense the medium to sterile screw-capped test tubes (typically 12 ml medium into 125 by 15 mm test tubes) under air and cap. Allow to stand until the medium is solidified and a O2-gradient formed (as evidenced by a pink upper oxic zone and a colorless lower anoxic zone). Inoculate cells just below the pink-colorless interface in the medium tube using a syringe.
6. Use trace minerals (see Medium No. 151) with final 0.4 g/L Na2MoO4 x 2H2O, 0.02 g/L CuSO4 x 5H2O and 0.01 g/L NiCl2 x 6H2O.
7. Comment: The medium tubes can be stocked under a N2 gas atmosphere. In this case, prior to use, fill the head space with air and allow to stand until the O2 gradient is formed.

Target organisms

No target organisms are recorded.

Provenance