thermoplasma_acidophilum_medium

CultureMech:003264 archaea COMPLEX LIQUID
Original name: THERMOPLASMA ACIDOPHILUM MEDIUM

Overview

External ontology
mediadive.medium:J917 (source link unavailable) — THERMOPLASMA ACIDOPHILUM MEDIUM
Applications
  • Microbial cultivation
pH
2.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=917

Composition

flowchart LR
    CultureMech_003264["thermoplasma_acidophilum_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003264 --> ing0_Yeast_extract
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_003264 --> ing1_Casamino_acids
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003264 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003264 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003264 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_003264 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003264 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Yeast extract 1 G_PER_L
2 Casamino acids 1 G_PER_L
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
0.2 G_PER_L CHEBI:26710
sodium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate

Preparation

  1. Adjust pH to 2.0 with 10 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.998188Z mediadive-import Imported from MediaDive Source: JCM, ID: J917
2026-01-28T19:48:24.116249Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:19.034718Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003264
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.939365+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOPLASMA ACIDOPHILUM MEDIUM" to "thermoplasma_acidophilum_medium" for consistent matching
2026-06-05T07:40:17.905690+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.459271+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:43.174352+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.