peat_medium_2_for_methanobacteria

CultureMech:003271 archaea COMPLEX LIQUID
Original name: PEAT MEDIUM 2 FOR METHANOBACTERIA

Overview

External ontology
mediadive.medium:J924 (source link unavailable) — PEAT MEDIUM 2 FOR METHANOBACTERIA
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=924

Composition

flowchart LR
    CultureMech_003271["peat_medium_2_for_methanobacteria"]
    ing0_MES["MES"]
    CultureMech_003271 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003271 --> ing1_Yeast_extract
    ing2_Coenzyme_M["Coenzyme M"]
    CultureMech_003271 --> ing2_Coenzyme_M
    CHEBI_17905["CHEBI:17905"]
    ing2_Coenzyme_M -.-> CHEBI_17905
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_003271 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003271 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_003271 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_KCl["KCl"]
    CultureMech_003271 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_KH2PO4["KH2PO4"]
    CultureMech_003271 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_NH4Cl["NH4Cl"]
    CultureMech_003271 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Tris_base["Tris base"]
    CultureMech_003271 --> ing9_Tris_base
    CHEBI_9754["CHEBI:9754"]
    ing9_Tris_base -.-> CHEBI_9754
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003271 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_TiCl3["TiCl3"]
    CultureMech_003271 --> ing11_TiCl3
    ing12_Biotin["Biotin"]
    CultureMech_003271 --> ing12_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_Biotin -.-> CHEBI_15956
    ing13_Folic_acid["Folic acid"]
    CultureMech_003271 --> ing13_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing13_Folic_acid -.-> CHEBI_27470
    ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003271 --> ing14_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003271 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Riboflavin["Riboflavin"]
    CultureMech_003271 --> ing16_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing16_Riboflavin -.-> CHEBI_17015
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003271 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003271 --> ing18_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_003271 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003271 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_Lipoic_acid["Lipoic acid"]
    CultureMech_003271 --> ing21_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing21_Lipoic_acid -.-> CHEBI_16494
    ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003271 --> ing22_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing23_ZnCl2["ZnCl2"]
    CultureMech_003271 --> ing23_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing23_ZnCl2 -.-> CHEBI_49976
    ing24_H3BO3["H3BO3"]
    CultureMech_003271 --> ing24_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing24_H3BO3 -.-> CHEBI_33118
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003271 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003271 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing27_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003271 --> ing27_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing27_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing28_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003271 --> ing28_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing28_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing29_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003271 --> ing29_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing29_MgSO4_x_7_H2O -.-> CHEBI_31795
    CultureMech_003271 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 MES 0.1 G_PER_L CHEBI:39010
MES
2 Yeast extract 0.01 G_PER_L
3 Coenzyme M
C2H6O3S2 142.201 g/mol
0.025 G_PER_L CHEBI:17905
coenzyme M
4 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.015 G_PER_L CHEBI:32954
sodium acetate
5 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.05 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
6 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
37.23 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
7 KCl
Cl.K 74.551 g/mol
0.15 G_PER_L CHEBI:32588
potassium chloride
8 KH2PO4
H2O4P.K 136.084 g/mol
1.36 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 NH4Cl
Cl.H4N 53.492 g/mol
2.68 G_PER_L CHEBI:31206
ammonium chloride
10 Tris base
C4H11NO3 121.136 g/mol
7.2 G_PER_L CHEBI:9754
tris
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
4.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
 ( disodium salt)
12 TiCl3 0.55 G_PER_L
13 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
14 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
15 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
18 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
19 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
20 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
23 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
24 ZnCl2
2Cl.Zn 136.296 g/mol
0.075 G_PER_L CHEBI:49976
zinc dichloride
25 H3BO3
H3BO3 61.833 g/mol
0.019 G_PER_L CHEBI:33118
boric acid
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
27 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
28 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.344 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
29 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.026 G_PER_L CHEBI:86360
manganese(II) sulfate
30 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.556 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
31 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.336 G_PER_L CHEBI:86158
calcium chloride dihydrate
32 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.009 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
33 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
3.446 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate

Preparation

  1. Mix components thoroughly, bring to a boil for 5 min, then, cool down under a stream of a N2-CO2 (80:20, v/v) gas mixture. Distribute the medium into Balch-type culture tubes (5 ml each) under the same gas mixture, seal with thick blue butyl rubber stoppers and autoclave.
  2. To complete the medium, add the following solutions to each of culture tubes (containing 5 ml medium) from sterile anaerobic stocks (autoclaved or *filter-sterilized):
  3. Pressurize the tubes to 70 kPa H2-CO2 (80:20, v/v) and allow to stand for at least 7 hr. Check the final pH of the medium to be about 5.7.
  4. Adjust pH to 7.0 with NaOH.
  5. Filter-sterilize and store under N2 at 4C in the dark.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.020336Z mediadive-import Imported from MediaDive Source: JCM, ID: J924
2026-02-06T05:10:23.884765Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:19.098555Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003271
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.009625+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PEAT MEDIUM 2 FOR METHANOBACTERIA" to "peat_medium_2_for_methanobacteria" for consistent matching
2026-06-05T07:41:41.082558+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.940185+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.