mrs_medium_with_soybean_peptone

CultureMech:003272 bacterial COMPLEX SOLID_AGAR
Original name: MRS MEDIUM WITH SOYBEAN PEPTONE

Overview

External ontology
mediadive.medium:J925 (source link unavailable) — MRS MEDIUM WITH SOYBEAN PEPTONE
Applications
  • Microbial cultivation
Notes
JCM Medium 925 MRS Medium With Soybean Peptone lists 55.0 g/L Lactobacilli MRS broth, 5.0 g/L Phytone peptone, 15.0 g/L Bacto agar, and 1.0 L Distilled water; the JCM page applies default autoclaving at 121 C for 15 min.

Composition

flowchart LR
    CultureMech_003272["mrs_medium_with_soybean_peptone"]
    ing0_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
    CultureMech_003272 --> ing0_Lactobacilli_MRS_broth__BD_Difco_
    ing1_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
    CultureMech_003272 --> ing1_Phytone_peptone__BD_BBL_
    ing2_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_003272 --> ing2_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing2_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_003272 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Lactobacilli MRS broth (BD-Difco) 55.0 G_PER_L TOGO M971 / JCM Medium 925 lists 55.0 g/L Lactobacilli MRS broth (BD-Difco).
2 Phytone peptone (BD-BBL) 5.0 G_PER_L FOODON:03315720
Soy peptone
 TOGO M971 / JCM Medium 925 lists 5.0 g/L Phytone peptone (BD-BBL).
3 Bacto agar (BD-Difco) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M971 / JCM Medium 925 lists 15.0 g/L Bacto agar (BD-Difco).
4 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M971 / JCM Medium 925 lists 1.0 L Distilled water.

Preparation

  1. Combine the MRS Medium With Soybean Peptone components listed by JCM Medium 925.
  2. Autoclave at 121 C for 15 min unless otherwise stated.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.026072Z mediadive-import Imported from MediaDive Source: JCM, ID: J925
2026-03-13T06:19:19.105133Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003272
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.017679+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS MEDIUM WITH SOYBEAN PEPTONE" to "mrs_medium_with_soybean_peptone" for consistent matching
2026-06-05T07:41:38.530438+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m971_mrs_soybean_score15.py RESOLVED_JCM_J925_MRS_SOYBEAN Restored the 1.0 L distilled-water row, normalized the JCM Medium 925 ingredient names to their source-listed commercial BD product names, added JCM autoclave evidence, removed the stale MediaDive 12 match, and linked the TOGO M971 source duplicate.