methylobacter_mr1_medium

CultureMech:003275 bacterial DEFINED LIQUID
Original name: METHYLOBACTER MR1 MEDIUM

Overview

External ontology
mediadive.medium:J928 (source link unavailable) — METHYLOBACTER MR1 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=928

Composition

flowchart LR
    CultureMech_003275["methylobacter_mr1_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003275 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003275 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_003275 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003275 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003275 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003275 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_003275 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaNO3["NaNO3"]
    CultureMech_003275 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_NH4Cl["NH4Cl"]
    CultureMech_003275 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003275 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003275 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_NaHCO3["NaHCO3"]
    CultureMech_003275 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003275 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003275 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003275 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003275 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003275 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_003275 --> ing17_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing18_H3BO3["H3BO3"]
    CultureMech_003275 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003275 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003275 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003275 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Biotin["Biotin"]
    CultureMech_003275 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_Folic_acid["Folic acid"]
    CultureMech_003275 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003275 --> ing24_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing25_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003275 --> ing25_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing25_Thiamine_HCl -.-> CHEBI_49105
    ing26_Riboflavin["Riboflavin"]
    CultureMech_003275 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003275 --> ing27_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing27_Nicotinic_acid -.-> CHEBI_15940
    ing28_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003275 --> ing28_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing28_Calcium_pantothenate -.-> CHEBI_31345
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_003275 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_003275 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.9005 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.0895522 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2HPO4
HO4P.2Na 141.958 g/mol
0.258706 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.497512 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.38308 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.1791 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 KCl
Cl.K 74.551 g/mol
0.328358 G_PER_L CHEBI:32588
potassium chloride
8 NaNO3
NO3.Na 84.994 g/mol
0.248756 G_PER_L CHEBI:63005
sodium nitrate
9 NH4Cl
Cl.H4N 53.492 g/mol
0.248756 G_PER_L CHEBI:31206
ammonium chloride
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.11990050000000001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.0199005, 0.1]
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
12 NaHCO3
CHO3.Na 84.006 g/mol
1.25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.02 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.005 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
24 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
25 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
26 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
28 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
29 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
30 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
32 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components, adjust pH to 6.6 - 6.8 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
  2. Distribute the medium into culture vessels (leave >80% volume gas phase), purge air in the gas phase with N2 for 1 min and seal with butyl rubber stoppers. Inject O2 gas into the gas phase (1.5% volume of the gas phase). Then, overpressure the culture vessels with methane gas (50% volume of the gas phase).
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then, proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.030654Z mediadive-import Imported from MediaDive Source: JCM, ID: J928
2026-03-13T03:09:23.389309Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:19.120682Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003275
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.034015+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOBACTER MR1 MEDIUM" to "methylobacter_mr1_medium" for consistent matching
2026-06-05T07:41:33.010071+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.511016+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.