9k_medium

CultureMech:003277 bacterial DEFINED LIQUID
Original name: 9K MEDIUM

Overview

External ontology
mediadive.medium:J92 (source link unavailable) — 9K MEDIUM
Applications
  • Microbial cultivation
pH
3.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=92

Composition

flowchart LR
    CultureMech_003277["9k_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_003277 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003277 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_KCl["KCl"]
    CultureMech_003277 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003277 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003277 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_003277 --> ing5_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing5_Ca_NO3_2 -.-> CHEBI_64205
    ing6_H2SO4["H2SO4"]
    CultureMech_003277 --> ing6_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing6_H2SO4 -.-> CHEBI_26836

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.499501 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.997 G_PER_L CHEBI:62946
ammonium sulfate
3 KCl
Cl.K 74.551 g/mol
0.0999001 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.499501 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
49.9501 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.00999001 G_PER_L CHEBI:64205
calcium nitrate
7 H2SO4
H2O4S 98.079 g/mol
1 G_PER_L CHEBI:26836
sulfuric acid

Preparation

  1. Adjust pH to 3.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.716491Z mediadive-import Imported from MediaDive Source: JCM, ID: J92
2026-02-06T05:10:41.566544Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:19.139420Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003277
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.055479+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "9K MEDIUM" to "9k_medium" for consistent matching
2026-05-24T04:38:33.227537+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=2 env(s) from CommunityMech
2026-06-05T07:40:15.983714+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.194785+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.591874+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.