mrs_medium_with_2_5_nacl_ph_9_0

CultureMech:003279 bacterial COMPLEX SOLID_AGAR
Original name: MRS MEDIUM WITH 2.5% NaCl (pH 9.0)

Overview

External ontology
mediadive.medium:J931 (source link unavailable) — MRS MEDIUM WITH 2.5% NaCl (pH 9.0)
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=931

Composition

flowchart LR
    CultureMech_003279["mrs_medium_with_2_5_nacl_ph_9_0"]
    ing0_Lactobacilli_MRS_broth["Lactobacilli MRS broth"]
    CultureMech_003279 --> ing0_Lactobacilli_MRS_broth
    ing1_NaCl["NaCl"]
    CultureMech_003279 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Agar["Agar"]
    CultureMech_003279 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Lactobacilli MRS broth 55 G_PER_L
2 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
3 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 9.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.041473Z mediadive-import Imported from MediaDive Source: JCM, ID: J931
2026-03-13T06:19:19.145432Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003279
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.062951+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS MEDIUM WITH 2.5% NaCl (pH 9.0)" to "mrs_medium_with_2_5_nacl_ph_9_0" for consistent matching
2026-06-05T07:41:38.517012+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.