oscillibacter_gh_medium (CultureMech:003280)

Overview

External ontology

mediadive.medium:J932 (source link unavailable) — OSCILLIBACTER GH MEDIUM

Applications

Microbial cultivation

pH

6.0

Notes

JCM Medium 932 lists 5.0 g yeast extract from BD-Difco, 5.0 g Polypeptone peptone from BD-BBL, 0.03 g Tween 80, 10.0 ml Trace vitamins from JCM Medium 197, 1.0 ml FeCl2 solution from JCM Medium 187, 1.0 ml Trace element solution from JCM Medium 187, and 1.0 L distilled water; after mixing, adjust pH to 6.0, distribute under N2, seal with butyl rubber stoppers, and autoclave.

Composition

flowchart LR
    CultureMech_003280["oscillibacter_gh_medium"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_003280 --> ing0_Yeast_extract__BD_Difco_
    ing1_Polypeptone_peptone__BD_BBL_["Polypeptone peptone (BD-BBL)"]
    CultureMech_003280 --> ing1_Polypeptone_peptone__BD_BBL_
    ing2_Tween_80["Tween 80"]
    CultureMech_003280 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Distilled_water["Distilled water"]
    CultureMech_003280 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

#	Ingredient	Concentration	Ontology	Notes
1	Yeast extract (BD-Difco)	4.94071 G_PER_L	FOODON:03315426 yeast extract	MediaDive J932 / JCM Medium 932 lists 4.94071 g/L Yeast extract (BD-Difco).
2	Polypeptone peptone (BD-BBL)	4.94071 G_PER_L	FOODON:03315306 Polypeptone	MediaDive J932 / JCM Medium 932 lists 4.94071 g/L Polypeptone peptone (BD-BBL).
3	Tween 80 (C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol	0.0296443 G_PER_L	CHEBI:53426 polysorbate 80	MediaDive J932 / JCM Medium 932 lists 0.0296443 g/L Tween 80.
4	Distilled water H2O 18.015 g/mol	988.142 ML_PER_L	CHEBI:15377 water	MediaDive J932 / JCM Medium 932 makes 1.012 L medium from 1.0 L distilled water plus 12.0 ml stock additions, equivalent to 988.142 ml/L distilled water.

Preparation

Mix yeast extract, Polypeptone peptone, Tween 80, distilled water, 9.88142 ml/L Trace vitamins, 0.988142 ml/L FeCl2 solution, and 0.988142 ml/L Trace element solution.
Adjust pH to 6.0.
Distribute the medium into culture vessels under a N2 gas stream and seal with butyl rubber stoppers.
Autoclave the sealed medium.

Solutions

Trace vitamins

Dose: 9.88142 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Biotin	2.0 MG_PER_L	JCM Medium 197 lists 2.0 mg/L Biotin.
Folic acid	2.0 MG_PER_L	JCM Medium 197 lists 2.0 mg/L Folic acid.
Pyridoxine hydrochloride	10.0 MG_PER_L	JCM Medium 197 lists 10.0 mg/L Pyridoxine hydrochloride.
Thiamine HCl	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L Thiamine HCl.
Riboflavin	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L Riboflavin.
Nicotinic acid	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L Nicotinic acid.
Calcium pantothenate	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L Calcium pantothenate.
Vitamin B12	0.1 MG_PER_L	JCM Medium 197 lists 0.1 mg/L Vitamin B12.
p-Aminobenzoic acid	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid.
Lipoic acid	5.0 MG_PER_L	JCM Medium 197 lists 5.0 mg/L Lipoic acid.
Distilled water	1000.0 ML_PER_L	JCM Medium 197 lists 1000.0 ml/L Distilled water.

MediaDive J932 / JCM Medium 932 adds 9.88142 ml/L Trace vitamins.

FeCl2 solution

Dose: 0.988142 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
HCl	2.5 G_PER_L	JCM Medium 187 lists 2.5 g/L HCl.
FeCl2 x 4 H2O	1.5 G_PER_L	JCM Medium 187 lists 1.5 g/L FeCl2 x 4 H2O.
Distilled water	990.0 ML_PER_L	JCM Medium 187 lists 990.0 ml/L Distilled water.

MediaDive J932 / JCM Medium 932 adds 0.988142 ml/L FeCl2 solution.

Trace element solution

Dose: 0.988142 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
ZnCl2	70.0 MG_PER_L	JCM Medium 187 lists 70.0 mg/L ZnCl2.
MnCl2 x 4 H2O	100.0 MG_PER_L	JCM Medium 187 lists 100.0 mg/L MnCl2 x 4 H2O.
H3BO3	6.0 MG_PER_L	JCM Medium 187 lists 6.0 mg/L H3BO3.
CoCl2 x 6 H2O	190.0 MG_PER_L	JCM Medium 187 lists 190.0 mg/L CoCl2 x 6 H2O.
CuCl2 x 2 H2O	2.0 MG_PER_L	JCM Medium 187 lists 2.0 mg/L CuCl2 x 2 H2O.
NiCl2 x 6 H2O	24.0 MG_PER_L	JCM Medium 187 lists 24.0 mg/L NiCl2 x 6 H2O.
Na2MoO4 x 2 H2O	36.0 MG_PER_L	JCM Medium 187 lists 36.0 mg/L Na2MoO4 x 2 H2O.
Distilled water	1000.0 ML_PER_L	JCM Medium 187 lists 1000.0 ml/L Distilled water.

MediaDive J932 / JCM Medium 932 adds 0.988142 ml/L Trace element solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:18.042989Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J932
2026-03-13T06:19:19.152927Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003280
2026-03-17T06:30:29.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.071663+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "OSCILLIBACTER GH MEDIUM" to "oscillibacter_gh_medium" for consistent matching
2026-06-05T07:41:40.630912+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.813870+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m978_oscillibacter_score15.py	RESOLVED_MEDIADIVE_J932_OSCILLIBACTER_GH	Restored the JCM Medium 932 source formula, added the missing water row, moved Trace vitamins, FeCl2 solution, and Trace element solution from flattened direct ingredients into nested ml/L solution additions, and linked the TOGO M978 source duplicate.