Overview
- External ontology
- mediadive.medium:J934 (source link unavailable) — ACIDILOBUS SACCHAROVORANS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=934
Composition
flowchart LR
CultureMech_003282["acidilobus_saccharovorans_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_003282 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_003282 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_KCl["KCl"]
CultureMech_003282 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003282 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003282 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Yeast_extract["Yeast extract"]
CultureMech_003282 --> ing5_Yeast_extract
ing6_Sulfur["Sulfur"]
CultureMech_003282 --> ing6_Sulfur
CHEBI_26833["CHEBI:26833"]
ing6_Sulfur -.-> CHEBI_26833
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003282 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Resazurin["Resazurin"]
CultureMech_003282 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_HCl["HCl"]
CultureMech_003282 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003282 --> ing10_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
ing11_ZnCl2["ZnCl2"]
CultureMech_003282 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003282 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_003282 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003282 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003282 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003282 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003282 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Biotin["Biotin"]
CultureMech_003282 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_003282 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003282 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_003282 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_003282 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_003282 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003282 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_003282 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003282 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Lipoic_acid["Lipoic acid"]
CultureMech_003282 --> ing27_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_Lipoic_acid -.-> CHEBI_16494
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.326087 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 308 |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.326087 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 308 |
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.326087 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 308 |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.326087 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Copied from referenced JCM Medium 308 |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.326087 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 308 |
| 6 | Yeast extract | 0.296443 G_PER_L | Copied from referenced JCM Medium 308 | |
| 7 |
Sulfur
S 32.067 g/mol
|
9.88142 G_PER_L |
CHEBI:26833
sulfur atom
|
Copied from referenced JCM Medium 308 |
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.494071 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 308 |
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000988142 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 308 |
| 10 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Copied from referenced JCM Medium 308 |
| 11 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Copied from referenced JCM Medium 308 |
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
Copied from referenced JCM Medium 308 |
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 308 |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 308 |
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Copied from referenced JCM Medium 308 |
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Copied from referenced JCM Medium 308 |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
Copied from referenced JCM Medium 308 |
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 308 |
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
Copied from referenced JCM Medium 308 |
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
Copied from referenced JCM Medium 308 |
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Copied from referenced JCM Medium 308 |
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Copied from referenced JCM Medium 308 |
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
Copied from referenced JCM Medium 308 |
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
Copied from referenced JCM Medium 308 |
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Copied from referenced JCM Medium 308 |
| 26 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
Copied from referenced JCM Medium 308 |
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Copied from referenced JCM Medium 308 |
| 28 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Copied from referenced JCM Medium 308 |
Preparation
- Use JCM medium No. 308 supplemented with 0.1 g/L (final) yeast extract and 2.0 g/L glucose (separately autoclaved).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.051906Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J934 |
| 2026-02-03T11:26:56.673876 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:19.173166Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003282 |
| 2026-03-17T19:45:33.094253+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDILOBUS SACCHAROVORANS MEDIUM" to "acidilobus_saccharovorans_medium" for consistent matching |
| 2026-04-04T21:48:41.426187 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from acidilobus_medium (CultureMech:002665) |
| 2026-04-04T22:03:18.701426 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from acidilobus_medium (CultureMech:002665) |
| 2026-05-16T08:57:22.242853+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:47.286751+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.587624+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |