fervidicoccus_fontis_medium

CultureMech:003283 archaea COMPLEX LIQUID
Original name: FERVIDICOCCUS FONTIS MEDIUM

Overview

External ontology
mediadive.medium:J935 (source link unavailable) — FERVIDICOCCUS FONTIS MEDIUM
Applications
  • Microbial cultivation
pH
5.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=935

Composition

flowchart LR
    CultureMech_003283["fervidicoccus_fontis_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003283 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003283 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_003283 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003283 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003283 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_NaCl["NaCl"]
    CultureMech_003283 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NaHCO3["NaHCO3"]
    CultureMech_003283 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Resazurin["Resazurin"]
    CultureMech_003283 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.315186 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.315186 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.315186 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.420248 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.668577 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 NaCl
Cl.Na 58.443 g/mol
0.477555 G_PER_L CHEBI:26710
sodium chloride
7 NaHCO3
CHO3.Na 84.006 g/mol
0.764088 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Resazurin
C12H7NO4 229.191 g/mol
0.00095511 G_PER_L CHEBI:8806
Resazurin

Preparation

  1. Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
  2. Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary.

Solutions

FeCl2 solution

Dose: 1 ML_PER_L

Trace element solution

Dose: 1 ML_PER_L

10% (w/v) Yeast extract (BD-Difco) solution

Dose: 5 ML_PER_L

MediaDive source attribute: w/v) Yeast extract (BD-Difco, 10%

10% (w/v) Trypticase peptone (BD-BBL) solution

Dose: 20 ML_PER_L

MediaDive source attribute: w/v) Trypticase peptone (BD-BBL, 10%

Trace vitamins

Dose: 10 ML_PER_L

MediaDive source attribute: *

5% (w/v) Na2S x 9 H2O solution

Dose: 10 ML_PER_L

MediaDive source attribute: w/v, 5%

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-26T17:53:18.053613Z mediadive-import Imported from MediaDive Source: JCM, ID: J935
2026-03-13T03:09:39.447638Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:19.183399Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003283
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.104519+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FERVIDICOCCUS FONTIS MEDIUM" to "fervidicoccus_fontis_medium" for consistent matching
2026-06-05T07:41:00.281146+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.835756+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-25T00:00:00-07:00 repair_mediadive_jcm_structure.py RESTORED_STOCK_SOLUTION_BOUNDARIES Rebuilt J935 from the checked-in MediaDive API primary recipe and JCM GRMD 935. Removed stock-strength components flattened into final ingredients and restored each stock's asserted ml/L addition volume.