mb_medium_for_methanobacterium

CultureMech:003293 archaea DEFINED LIQUID
Original name: MB MEDIUM FOR METHANOBACTERIUM

Overview

External ontology
mediadive.medium:J945 (source link unavailable) — MB MEDIUM FOR METHANOBACTERIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=945

Composition

flowchart LR
    CultureMech_003293["mb_medium_for_methanobacterium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003293 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003293 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_003293 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003293 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003293 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003293 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Resazurin["Resazurin"]
    CultureMech_003293 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_003293 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003293 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003293 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003293 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003293 --> ing11_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing11_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003293 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003293 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003293 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003293 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_H3BO3["H3BO3"]
    CultureMech_003293 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003293 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_Biotin["Biotin"]
    CultureMech_003293 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Folic_acid["Folic acid"]
    CultureMech_003293 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003293 --> ing20_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing21_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003293 --> ing21_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing21_Thiamine_HCl -.-> CHEBI_49105
    ing22_Riboflavin["Riboflavin"]
    CultureMech_003293 --> ing22_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing22_Riboflavin -.-> CHEBI_17015
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003293 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003293 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_003293 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003293 --> ing26_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing27_Lipoic_acid["Lipoic acid"]
    CultureMech_003293 --> ing27_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27_Lipoic_acid -.-> CHEBI_16494

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.148221 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.296443 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
0.592885 G_PER_L CHEBI:26710
sodium chloride
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.296443 G_PER_L CHEBI:62946
ammonium sulfate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.118577 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.118577, 3.0]
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0790514 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Resazurin
C12H7NO4 229.191 g/mol
0.000988142 G_PER_L CHEBI:8806
Resazurin
8 NaHCO3
CHO3.Na 84.006 g/mol
48 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
20 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
21 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
22 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
23 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
26 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
27 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
28 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (filter-sterilized):
  2. Distribute the medium into culture vessels (leave >80% gas phase by volume) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
  3. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.087034Z mediadive-import Imported from MediaDive Source: JCM, ID: J945
2026-03-13T03:09:41.187076Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:19.269229Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003293
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.200232+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MB MEDIUM FOR METHANOBACTERIUM" to "mb_medium_for_methanobacterium" for consistent matching
2026-06-05T07:41:13.279000+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.014568+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.